Abstract
A rapid and simple method for the determination of rubella immunity is described. The method, which employs passive hemolysis in agarose gel, is sensitive and reproducible and does not require prior absorption of test sera to remove inhibitors. Immunoglobulin G, but not immunoglobulin M, antibodies were regularly found to give demonstrable reactions in the test. It is concluded that the hemolysis-in-gel test may provide a valuable tool, in particular for mass screening for rubella immunity.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hall J. M. Specificity of antibody formation after intravitreal immunization with bovine gamma globulin and ovalbumin. I. Primary response. Invest Ophthalmol. 1971 Oct;10(10):775–783. [PubMed] [Google Scholar]
- Halonen P. E., Ryan J. M., Stewart J. A. Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proc Soc Exp Biol Med. 1967 May;125(1):162–167. doi: 10.3181/00379727-125-32038. [DOI] [PubMed] [Google Scholar]
- Jackson W. B., O'Connor G. R., Hall J. M. Plate hemolysin test for the rapid screening of toxoplasma antibodies. Appl Microbiol. 1974 May;27(5):896–900. doi: 10.1128/am.27.5.896-900.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Quirin E. P., Nelson D. B., Inhorn S. L. Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing. Appl Microbiol. 1972 Sep;24(3):353–357. doi: 10.1128/am.24.3.353-357.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]