Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Aug;8(2):134–141. doi: 10.1128/jcm.8.2.134-141.1978

Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassay which uses human immunoglobulin G in antigen-antibody complexes.

B Ziola, O Meurman, M T Matikainen, A Salmi, J L Kalliomäki
PMCID: PMC275170  PMID: 701459

Abstract

A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF.

Full text

PDF
134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. 1958 REVISION of diagnostic criteria for rheumatoid arthritis. Arthritis Rheum. 1959 Feb;2(1):16–20. doi: 10.1002/1529-0131(195902)2:1<16::aid-art1780020104>3.0.co;2-9. [DOI] [PubMed] [Google Scholar]
  2. AHO K. The problem of the antibody nature of the rheumatoid factor. Ann Med Exp Biol Fenn. 1961;39(Suppl 7):1–60. [PubMed] [Google Scholar]
  3. Arstila P., Vuorimaa T., Kalimo K., Halonen P., Viljanen M., Granfors K., Toivanen P. A solid-phase radioimmunoassay for IgG and IgM antibodies against measles virus. J Gen Virol. 1977 Jan;34(1):167–176. doi: 10.1099/0022-1317-34-1-167. [DOI] [PubMed] [Google Scholar]
  4. Cambiaso C. L., Goffinet A., Vaerman J. P., Heremans J. F. Glutaraldehyde-activated aminohexyl- derivative of Sepharose 4B as a new verstile immunoabsorbent. Immunochemistry. 1975 Apr;12(4):273–278. doi: 10.1016/0019-2791(75)90175-5. [DOI] [PubMed] [Google Scholar]
  5. Carson D. A., Lawrance S., Catalano M. A., Vaughan J. H., Abraham G. Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG. J Immunol. 1977 Jul;119(1):295–300. [PubMed] [Google Scholar]
  6. Focan-Henrard D., Franchimont P., Bertrand E. Radioimmunoassay of the rheumatoid factors. Rheumatology. 1975;6:140–150. [PubMed] [Google Scholar]
  7. Franchimont P., Suteanu S. Radioimmunoassay of rheumatoid factor. Arthritis Rheum. 1969 Oct;12(5):483–490. doi: 10.1002/art.1780120504. [DOI] [PubMed] [Google Scholar]
  8. HARBOE M. Relation between Gm types and hemagglutinating substances in rheumatoid sera. Acta Pathol Microbiol Scand. 1960;50:89–105. doi: 10.1111/j.1699-0463.1960.tb01175.x. [DOI] [PubMed] [Google Scholar]
  9. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  10. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson P. M., Faulk W. P. Rheumatoid factor: its nature, specificity, and production in rheumatoid arthritis. Clin Immunol Immunopathol. 1976 Nov;6(3):414–430. doi: 10.1016/0090-1229(76)90094-5. [DOI] [PubMed] [Google Scholar]
  12. Kalimo K. O., Meurman O. H., Halonen P. E., Ziola B. R., Viljanen M. K., Granfors K., Toivanen P. Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol. 1976 Aug;4(2):117–123. doi: 10.1128/jcm.4.2.117-123.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kalimo K. O., Ziola B. R., Viljanen M. K., Granfors K., Toivanen P. Solid-phase radioimmunoassay of herpes simplex virus IgG and IgM antibodies. J Immunol Methods. 1977;14(2):183–195. doi: 10.1016/0022-1759(77)90008-4. [DOI] [PubMed] [Google Scholar]
  14. Knez V., Reimer C. B. An indirect solid-phase microradioimmunoassay for human IgM-anti-IgG (rheumatoid factor). J Immunol Methods. 1977;18(1-2):105–121. doi: 10.1016/0022-1759(77)90162-4. [DOI] [PubMed] [Google Scholar]
  15. Kochwa S., Brownell M., Rosenfield R. E., Wasserman L. R. Adsorption of proteins by polystyrene particles. I. Molecular unfolding and acquired immunogenicity of IgG. J Immunol. 1967 Nov;99(5):981–986. [PubMed] [Google Scholar]
  16. Krohn K., Sherman L., Welch M. Studies of radioiodinated fibrinogen. I. Physicochemical properties of the ICl, chloramine-T, and electrolytic reaction products. Biochim Biophys Acta. 1972 Dec 28;285(2):404–413. doi: 10.1016/0005-2795(72)90327-3. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Levine S., Hamilton R. Kinetics of the respiratory syncytial virus growth cycle in HeLa cells. Arch Gesamte Virusforsch. 1969;28(2):122–132. doi: 10.1007/BF01249378. [DOI] [PubMed] [Google Scholar]
  19. Meurman O. H., Viljanen M. K., Granfors K. Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test. J Clin Microbiol. 1977 Mar;5(3):257–262. doi: 10.1128/jcm.5.3.257-262.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nineham L. J., Hay F. C., Roitt I. M. Laboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and IgM antiglobulins. J Clin Pathol. 1976 Dec;29(12):1121–1126. doi: 10.1136/jcp.29.12.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Normansell D. E. Anti- -globulins in rheumatoid arthritis sera. 3. The reactivity of anti- -globulin rheumatoid factors with heterologous G-globulin. Immunochemistry. 1972 Jul;9(7):725–736. doi: 10.1016/0019-2791(72)90016-x. [DOI] [PubMed] [Google Scholar]
  22. SIEVERS K. THE RHEUMATOID FACTOR IN DEFINITE RHEUMATOID ARTHRITIS. AN ANALYSIS OF 1279 ADULT PATIENTS, WITH A FOLLOW-UP STUDY. Acta Rheumatol Scand Suppl. 1965:SUPPL 9–121. doi: 10.3109/rhe1.1965.10.suppl-9.01. [DOI] [PubMed] [Google Scholar]
  23. Shirodaria P. V., Fraser K. B., Stanford F. Secondary fluorescent staining of virus antigens by rheumatoid factor and fluorescein-conjugated anti-IgM. Ann Rheum Dis. 1973 Jan;32(1):53–57. doi: 10.1136/ard.32.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WALLER M. V., VAUGHAN J. H. Use of anti-Rh sera for demonstrating agglutination activating factor in rheumatoid arthritis. Proc Soc Exp Biol Med. 1956 May;92(1):198–200. doi: 10.3181/00379727-92-22426. [DOI] [PubMed] [Google Scholar]
  25. Wunner W. H., Pringle C. R. Respiratory syncytial virus proteins. Virology. 1976 Aug;73(1):228–243. doi: 10.1016/0042-6822(76)90077-5. [DOI] [PubMed] [Google Scholar]
  26. Ziola B. R., Matikainen M. T., Salmi A. Polystyrene balls as the solid-phase of a double-antibody radioimmunoassay for human serum albumin. J Immunol Methods. 1977;17(3-4):309–317. doi: 10.1016/0022-1759(77)90113-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES