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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Sep;8(3):313–319. doi: 10.1128/jcm.8.3.313-319.1978

Carbon substrate utilization studies of some cultures of Alcaligenes denitrificans, Alcaligenes faecalis, and Alcaligenes odorans isolated from clinical specimens.

H R Rarick, P S Riley, R Martin
PMCID: PMC275238  PMID: 701468

Abstract

One hundred and sixty-two cultures of Alcaligenes species (A. denitrificans, A. faecalis, and A. odorans) of clinical origin were characterized by routine diagnostic and carbon substrate utilization techniques. The microorganisms were tested for their ability to utilize a total of 188 substrates. Substrate utilization was assayed by (i) growth stimulation and (ii) substrate alkalinization. The A. denitrificans and A. odorans cultures had unique substrate utilization profiles for each species. The A. faecalis isolates were redefined by colonial morphology into two biotypes: (i) biotype I, morphologically and biochemically similar to the A. denitrificans cultures and (ii) biotype II, morphologically similar to the A. odorans cultures.

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Selected References

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  1. Baumann P., Doudoroff M., Stanier R. Y. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968 May;95(5):1520–1541. doi: 10.1128/jb.95.5.1520-1541.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumann P., Doudoroff M., Stanier R. Y. Study of the Moraxella group. I. Genus Moraxella and the Neisseria catarrhalis group. J Bacteriol. 1968 Jan;95(1):58–73. doi: 10.1128/jb.95.1.58-73.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark W. A. A simplified Leifson flagella stain. J Clin Microbiol. 1976 Jun;3(6):632–634. doi: 10.1128/jcm.3.6.632-634.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilardi G. L. Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie Van Leeuwenhoek. 1973;39(2):229–242. doi: 10.1007/BF02578855. [DOI] [PubMed] [Google Scholar]
  5. HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
  6. Oberhofer T. R., Rowen J. W. Acetamide agar for differentiation of nonfermentative bacteria. Appl Microbiol. 1974 Oct;28(4):720–721. doi: 10.1128/am.28.4.720-721.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oberhofer T. R., Rowen J. W., Cunningham G. F. Characterization and identification of gram-negative, nonfermentative bacteria. J Clin Microbiol. 1977 Feb;5(2):208–220. doi: 10.1128/jcm.5.2.208-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Otto L. A., Pickett M. J. Rapid method for identification of gram-negative, nonfermentative bacilli. J Clin Microbiol. 1976 Jun;3(6):566–575. doi: 10.1128/jcm.3.6.566-575.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Riley P. S., Rarick H. R., Utter G. Preliminary observations on the application of the multiple inocula (replicator) method for carbon substrate utilization studies of Alcaligenes faecalis. J Clin Microbiol. 1977 Apr;5(4):485–487. doi: 10.1128/jcm.5.4.485-487.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Riley P. S., Tatum H. W., Weaver R. E. Pseudomonas putrefaciens isolates from clinical specimens. Appl Microbiol. 1972 Nov;24(5):798–800. doi: 10.1128/am.24.5.798-800.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]

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