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. 1978 Oct;8(4):438–444. doi: 10.1128/jcm.8.4.438-444.1978

Detection of cells producing surface antigen-specific antibody to influenza viruses.

C McLaren, G E Grubbs, F A Ennis
PMCID: PMC275268  PMID: 363742

Abstract

B cells producing antibodies to influenza virus antigens were detected and quantitated by a hemolytic plaque assay. Responses of mice after primary infection and immunization with influenza viruses were measured and compared with responses after secondary immunization. The B-cell responses were specific and differentiated between A and B influenza viruses and between different subtypes of A influenza viruses. Responses to closely related influenza A virus strains of the H3N2 subtype cross-reacted but could also be differentiated. Cells secreting antibody to either of the virus surface antigens (hemagglutinin and neuraminidase) could be separately enumerated. Evidence that immunoglobulin G- secreting cells are detected in the assay without the use of facilitating anti-immunoglobulin G sera is presented.

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Selected References

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