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. 1978 Nov;8(5):473–479. doi: 10.1128/jcm.8.5.473-479.1978

Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

G Stiffler-Rosenberg, H Fey
PMCID: PMC275282  PMID: 365877

Abstract

The enzyme-linked immunosorbent assay (ELISA) introduced for the detection of staphylococcal enterotoxins by Saunders et al., Simon and Terplan, and ourselves has proved to be a simple, reliable, and sensitive test. A new modification is described that uses polystyrene balls (diameter, 6 mm) coated individually with antibody against one of the toxins A, B, or C. In a single tube, 20 ml of the food extract was incubated with the three balls differently stained, which were then each tested for the uptake of enterotoxin by a competitive ELISA. A concentration of 0.1 ng or less of enterotoxin per ml can be measured, making tedious concentration procedures of the extracts superfluous. Culture supernatants and extracts from foods artificially or naturally contaminated with toxin were successfully examined. Cross-reactions did not occur, and nonspecific interfering substances did not create serious problems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergdoll M. S., Borja C. R., Avena R. M. Identification of a new enterotoxin as enterotoxin C. J Bacteriol. 1965 Nov;90(5):1481–1485. doi: 10.1128/jb.90.5.1481-1485.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borja C. R., Bergdoll M. S. Purification and partial characterization of enterotoxin C produced by Staphylococcus aureus strain 137. Biochemistry. 1967 May;6(5):1467–1473. doi: 10.1021/bi00857a032. [DOI] [PubMed] [Google Scholar]
  3. Bukovic J. A., Johnson H. M. Staphylococcal enterotoxin C: solid-phase radioimmunoassay. Appl Microbiol. 1975 Oct;30(4):700–701. doi: 10.1128/am.30.4.700-701.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  5. Engvall E. Quantitative enzyme immunoassay (ELISA) in microbiology. Med Biol. 1977 Aug;55(4):193–200. [PubMed] [Google Scholar]
  6. Fey H., Pfister H., Messerli J., Sturzenegger N., Grolimund F. Methods of isolation, purification and quantitation of bovine immunoglobulins: a technical review. Zentralbl Veterinarmed B. 1976 May;23(4):269–300. doi: 10.1111/j.1439-0450.1976.tb00682.x. [DOI] [PubMed] [Google Scholar]
  7. Fey H., Stiffler-Rosenberg G. Messung von Tetanus-Antitoxin beim Pferd mit dem Enzyme Linked Immuno Sorbent Assay (ELISA). Schweiz Arch Tierheilkd. 1977 Nov;119(11):437–446. [PubMed] [Google Scholar]
  8. Johnson H. M., Bukovic J. A., Kauffman P. E., Peeler J. T. Staphylococcal enterotoxin B: solid-phase radioimmunoassay. Appl Microbiol. 1971 Nov;22(5):837–841. doi: 10.1128/am.22.5.837-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson H. M., Bukovic J. A., Kauffmann P. E. Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food. Appl Microbiol. 1973 Sep;26(3):309–313. doi: 10.1128/am.26.3.309-313.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kato E., Khan M., Kujovich L., Bergdoll M. S. Production of enterotoxin a. Appl Microbiol. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kauffman P. E., Johnson H. M. Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay. Appl Microbiol. 1975 Jun;29(6):776–779. doi: 10.1128/am.29.6.776-779.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  14. Niskanen A., Lindroth S. Preparation of labeled staphylococcal enterotoxin A with high specific activity. Appl Environ Microbiol. 1976 Dec;32(6):735–740. doi: 10.1128/aem.32.6.735-740.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pober Z., Silverman G. J. Modified radioimmunoassay determination for staphylococcal enterotoxin B in foods. Appl Environ Microbiol. 1977 Mar;33(3):620–625. doi: 10.1128/aem.33.3.620-625.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reiser R., Conaway D., Bergdoll M. S. Detection of staphylococcal enterotoxin in foods. Appl Microbiol. 1974 Jan;27(1):83–85. doi: 10.1128/am.27.1.83-85.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robern H., Dighton M., Yano Y., Dickie N. Double-antibody radioimmunoassay for staphylococcal enterotoxin C2. Appl Microbiol. 1975 Oct;30(4):525–529. doi: 10.1128/am.30.4.525-529.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saunders G. C., Bartlett M. L. Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A. Appl Environ Microbiol. 1977 Nov;34(5):518–522. doi: 10.1128/aem.34.5.518-522.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  20. Schantz E. J., Roessler W. G., Woodburn M. J., Lynch J. M., Jacoby H. M., Silverman S. J., Gorman J. C., Spero L. Purification and some chemical and physical properties of staphylococcal enterotoxin A. Biochemistry. 1972 Feb 1;11(3):360–366. doi: 10.1021/bi00753a009. [DOI] [PubMed] [Google Scholar]
  21. Simon E., Terplan G. Nachweis von Staphylokokken Enterotoxin B mittels ELISA-Test. Zentralbl Veterinarmed B. 1977 Dec;24(10):842–844. [PubMed] [Google Scholar]
  22. Stiffler-Rosenberg G., Fey H. Messung von Tetanus-Antitoxin mit dem Enzyme-linked immunosorbent assay (ELISA). Schweiz Med Wochenschr. 1977 Aug 6;107(31):1101–1104. [PubMed] [Google Scholar]
  23. Wisdom G. B. Enzyme-immunoassay. Clin Chem. 1976 Aug;22(8):1243–1255. [PubMed] [Google Scholar]

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