Abstract
A solid-phase radioimmunoassay (RIA) procedure was compared with the indirect fluorescent antibody (IFA) test in a serological study of 76 female adults with urinary tract infections. Relative serum antibody activity was determined against patients' homologous infecting enterobacteria by RIA and IFA and against heterologous enterobacterial common antigen (Escherichia coli O14) by RIA. There was marked correlation between results of the IFA and RIA methods using the homologous system; 22 of 51 patients (43%) with pyelonephritis had significantly elevated serum antibody activity by both IFA (titers greater than or equal to 512) and RIA (binding ratio greater than or equal to 2.0) when compared with normal serum controls; three had significant antibody activity detectable by RIA only. Eighteen (72%) of 25 patients with pyelonephritis had RIA binding ratios of greater than or equal to 2.0 against their homologous bacterial isolates and the enterobacterial common antigen; an additional 6 patients had binding ratios of greater than or equal to 2.0 against the antigen only. All 25 patients with cystitis had low serum antibody levels by IFA and RIA when tested against their own isolate as well as enterobacterial common antigen. The RIA procedure was objective, quantitative, and less tedious to perform than IFA.
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- Ahlstedt S., Jodal U., Hanson L. A., Holmgren J. Quantitation of Escherichia coli O antibodies by direct and indirect agglutination in comparison with a radioimmunoassay. Int Arch Allergy Appl Immunol. 1975;48(4):445–451. doi: 10.1159/000231332. [DOI] [PubMed] [Google Scholar]
- Daugharty H., Warfield D. T., Davis M. L. Solid-phase radioimmunoassay of total and influenza-specific immunoglobulin G. Appl Microbiol. 1972 Feb;23(2):360–367. doi: 10.1128/am.23.2.360-367.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diaz F., Neter E. Antibody response to the common enterobacterial antigen of children with shigellosis, salmonellosis or urinary tract infection. Am J Med Sci. 1968 Jul;256(1):18–24. doi: 10.1097/00000441-196807000-00003. [DOI] [PubMed] [Google Scholar]
- Mäkelä P. H., Mayer H. Enterobacterial common antigen. Bacteriol Rev. 1976 Sep;40(3):591–632. doi: 10.1128/br.40.3.591-632.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanford B. A., Smith K. O. Radioimmunoassay procedure for quantitating bacterial antibody in human sera. J Immunol Methods. 1977;14(3-4):313–323. doi: 10.1016/0022-1759(77)90142-9. [DOI] [PubMed] [Google Scholar]
- Smith K. O., Gehle W. D. Magnetic transfer devices for use in solid-phase radioimmunoassays and enzyme-linked immunosorbent assays. J Infect Dis. 1977 Oct;136 (Suppl):S329–S336. doi: 10.1093/infdis/136.supplement_2.s329. [DOI] [PubMed] [Google Scholar]
- Thomas V. L., Forland M., Shelokov A. Antibody-coated bacteria in urinary tract infection. Kidney Int Suppl. 1975 Aug;4:S20–S22. [PubMed] [Google Scholar]
- Thomas V. L., Harris R. E., Gilstrap L. C., 3rd, Shelokov A. Antibody-coated bacteria in the urine of obstetrical patients with acute pyelonephritis. J Infect Dis. 1975 May;131 (Suppl):S57–S61. doi: 10.1093/infdis/131.supplement.s57. [DOI] [PubMed] [Google Scholar]
- Thomas V., Shelokov A., Forland M. Antibody-coated bacteria in the urine and the site of urinary-tract infection. N Engl J Med. 1974 Mar 14;290(11):588–590. doi: 10.1056/NEJM197403142901102. [DOI] [PubMed] [Google Scholar]
- WHANG H. Y., NETER E. STUDY OF HETEROGENETIC (KUNIN) ANTIBODIES IN SERUM OF HEALTHY SUBJECTS AND CHILDREN WITH ENTERIC AND URINARY TRACT INFECTIONS. J Pediatr. 1963 Sep;63:412–419. doi: 10.1016/s0022-3476(63)80429-1. [DOI] [PubMed] [Google Scholar]