Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Dec;8(6):643–647. doi: 10.1128/jcm.8.6.643-647.1978

Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae.

D C Yong, A Prytula
PMCID: PMC275318  PMID: 106068

Abstract

A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baron E. S., Saz A. K. Effect of types of media on the production of acid from glucose by so-called glucose-negative strains of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Mar;3(3):330–333. doi: 10.1128/jcm.3.3.330-333.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beck A., Fluker J. L., Platt D. J. Neisseria meningitidis in urogenital infection. Br J Vener Dis. 1974 Oct;50(5):367–369. doi: 10.1136/sti.50.5.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beno D. W., Devine L. F., Larson G. L. Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton broth. J Bacteriol. 1968 Aug;96(2):563–563. doi: 10.1128/jb.96.2.563-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown W. J. A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae. Health Lab Sci. 1976 Jan;13(1):54–58. [PubMed] [Google Scholar]
  5. Brown W. J. Modification of the rapid fermentation test for Neisseria gonorrhoeae. Appl Microbiol. 1974 Jun;27(6):1027–1030. doi: 10.1128/am.27.6.1027-1030.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Faur Y. C., Weisburd M. H., Wilson M. E. Carbohydrate fermentation plate medium for confirmation of Neisseria species. J Clin Microbiol. 1975 Mar;1(3):294–297. doi: 10.1128/jcm.1.3.294-297.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Faur Y. C., Weisburd M. H., Wilson M. E. Isolation of Neisseria meningitidis from the Genito-urinary tract and anal canal. J Clin Microbiol. 1975 Sep;2(3):178–182. doi: 10.1128/jcm.2.3.178-182.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fiumara N. J., Wise H. M., Jr, Many M. Gonorrheal pharyngitis. N Engl J Med. 1967 Jun 1;276(22):1248–1250. doi: 10.1056/NEJM196706012762208. [DOI] [PubMed] [Google Scholar]
  9. Hare M. J. Comparative assessment of microbiological methods for the diagnosis of gonorrhea in women. Br J Vener Dis. 1974 Dec;50(6):437–441. doi: 10.1136/sti.50.6.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iqbal Y. Gonococcal tonsillitis. Br J Vener Dis. 1971 Apr;47(2):144–145. doi: 10.1136/sti.47.2.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KELLOGG D. S., Jr, DEACON W. E. A NEW RAPID IMMUNOFLUORESCENT STAINING TECHNIQUE FOR IDENTIFICATION OF TREPONEMA PALLIDUM AND NEISSERIA GONORRHOEAE. Proc Soc Exp Biol Med. 1964 Apr;115:963–965. doi: 10.3181/00379727-115-29090. [DOI] [PubMed] [Google Scholar]
  12. Kellogg D. S., Jr, Turner E. M. Rapid fermentation confirmation of Neisseria gonorrhoeae. Appl Microbiol. 1973 Apr;25(4):550–552. doi: 10.1128/am.25.4.550-552.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morse S. A., Bartenstein L. Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):8–13. doi: 10.1128/jcm.3.1.8-13.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peacock W. L., Jr, Welch B. G., Martin J. E., Jr, Thayer J. D. Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. Public Health Rep. 1968 Apr;83(4):337–339. [PMC free article] [PubMed] [Google Scholar]
  16. Pollock H. M. Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory. J Clin Microbiol. 1976 Jul;4(1):19–21. doi: 10.1128/jcm.4.1.19-21.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reddick A. A simple carbohydrate fermentation test for identification of the pathogenic Neisseria. J Clin Microbiol. 1975 Jul;2(1):72–73. doi: 10.1128/jcm.2.1.72-73.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  19. Shtibel R., Toma S. Neisseria gonorrhoeae: evaluation of some methods used for carbohydrate utilization. Can J Microbiol. 1978 Feb;24(2):177–181. doi: 10.1139/m78-030. [DOI] [PubMed] [Google Scholar]
  20. Stacey P., Warner G. Identification of Neisseria gonorrhoea and Neisseria meningitidis by a carbohydrate disc technique. J Clin Pathol. 1973 Apr;26(4):303–304. doi: 10.1136/jcp.26.4.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor G. S., Jr, Keys R. J. Simplified sugar fermentation plate technique for identification of Neisseria gonorrhoeae. Appl Microbiol. 1974 Feb;27(2):416–417. doi: 10.1128/am.27.2.416-417.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wallace R., Ashton F., Charron F., Diena B. B. An improved sugar fermentation technique for the confirmation of Neisseria gonorrhoeae. Can J Public Health. 1975 May-Jun;66(3):251–252. [PubMed] [Google Scholar]
  23. White L. A., Kellogg D. S., Jr An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria. Health Lab Sci. 1965 Oct;2(4):238–241. [PubMed] [Google Scholar]
  24. Young H., Paterson I. C., McDonald D. R. Rapid carbohydrate utilization test for the identification of Neisseria gonorrhoeae. Br J Vener Dis. 1976 Jun;52(3):172–175. doi: 10.1136/sti.52.3.172. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES