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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Biochemistry. 2009 Sep 29;48(38):9076–9083. doi: 10.1021/bi9006385

TABLE 1.

Kinetic properties of purified recombinant Rubisco enzymes

Enzyme kcat (s−1) Ω a
(VcKo/VoKc)
Kca
(µM CO2)
Koa
(µM O2)
Ko/Kcb Vc/Vob KRuBPa
(µ M)
Wild type 4.9 ± 0.9 39 ± 2 186 ± 13   850 ± 66 4.6 8.5 27 ± 3
D103V 4.9 ± 0.3 37 ± 3 388 ± 37   866 ± 40 2.2 16.8 25 ± 2
A375V 0.6 ± 0.1 32 ± 3 143 ± 7 1088 ± 43 7.6 4.2 34 ± 3
D103V/A375V 3.1 ± 0.3 30 ± 2 294 ± 31 1153 ± 142 3.9 7.7 269 ± 33
a

Values are the means ± standard deviation (n−1) of three separate enzyme preparations.

b

Calculated values.