. Author manuscript; available in PMC: 2010 Sep 29.

Published in final edited form as: Biochemistry. 2009 Sep 29;48(38):9076–9083. doi: 10.1021/bi9006385

TABLE 1.

Kinetic properties of purified recombinant Rubisco enzymes

Enzyme	k_cat (s⁻¹)	Ω ^a (V_cK_o/V_oK_c)	K_c^a (µM CO₂)	K_o^a (µM O₂)	K_o/K_c^b	V_c/V_o^b	K_RuBP^a (µ M)
Wild type	4.9 ± 0.9	39 ± 2	186 ± 13	850 ± 66	4.6	8.5	27 ± 3
D103V	4.9 ± 0.3	37 ± 3	388 ± 37	866 ± 40	2.2	16.8	25 ± 2
A375V	0.6 ± 0.1	32 ± 3	143 ± 7	1088 ± 43	7.6	4.2	34 ± 3
D103V/A375V	3.1 ± 0.3	30 ± 2	294 ± 31	1153 ± 142	3.9	7.7	269 ± 33

Values are the means ± standard deviation (n−1) of three separate enzyme preparations.

Calculated values.