Figure 8.

Interferon-γ (IFN-γ) induces the association of protein kinase C-α (PKC-α) with signal transducer and activator of transcription 1 (STAT1). Adherent bone marrow-derived macrophages (BMDM) were incubated in the absence or presence of 100 U/ml of IFN-γ for the indicated periods of time. PKC-α was immunoprecipitated and the presence of STAT1 in immunoprecipitates was determined by Western blot analysis, as described in the Materials and methods. The blot was stripped and reprobed with an anti-PKC-α immunoglobulin (IgG2b) to ensure that equal amounts of PKC-α were immunoprecipitated in each sample. Similar results were obtained in two independent experiments.