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. 2009 Sep;128(1 Pt 2):e700–e706. doi: 10.1111/j.1365-2567.2009.03063.x

Figure 2.

Figure 2

Involvement of nuclear factor-κB (NF-κB) activation in lipopolysaccharide (LPS) -induced astrocyte elevated gene 1 (AEG-1) expression. (a) U937 cells were treated with 5 μM parthenolide as a NF-κB inhibitor for 10 hr (top). U937 cells were transfected with IκB dominant negative mutant or empty vector as a negative control and further cultured for 2 days (bottom). (b) U937 cells were pretreated with 25 μm LY294002 as a phosphoinositide 3-kinase (PI3K) inhibitor for 10 hr (top). U937 cells were transfected with Akt dominant negative mutant or empty vector as a negative control and cultured for 2 days (bottom). Those cells were further stimulated with LPS (100 ng/ml) for 1 hr and the expression of AEG-1 was analysed by immunoblotting.