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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Methods. 2009 May 4;48(4):346–352. doi: 10.1016/j.ymeth.2009.02.026

Figure 4. TTF2 is the main termination factor in HNE.

Figure 4

A. Termination activity of TTF2 in the defined system. Isolated EECs were left untreated or supplemented with recombinant TTF2 and elongation of isolated EECs was carried out for seven minutes in the absence or presence of 1 pmol of ssDNA (a 48-nt DNA oligo with random sequences). Then the supernatants were separated from the beads and the transcripts in both fractions were analyzed as described in the legend to Fig. 2. B. TTF2 causes transcription termination in the presence of HNE. Isolated EECs were supplemented with a whole or TTF2-depleted extract and elongation was first carried out under regular low salt conditions for indicated amounts of time (LSC) then the salt was raised to 250 mM and elongation was continued for another seven minutes (HSC). The template used in this experiment generates 183 nt run-off transcripts. Transcripts were analyzed as described in the legend to figure 2.