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. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Nature. 2009 Aug 23;461(7261):230–235. doi: 10.1038/nature08283

Figure 5. Production of RMRP-derived endogenous siRNAs depends on Dicer.

Figure 5

a, Effect of suppressing Dicer on RMRP-derived small RNAs. Northern blotting was performed to detect [1] small RNAs using the antisense strand of RMRP as a probe in the indicated cells expressing control shRNA (sh-GFP) or Dicer-specific shRNAs (sh-Dicer #1 and sh-Dicer #2), [2] pre-miR-16 and mature miR-16 using a miR-16 specific probe, and [3] U6 RNA. See Supplementary Fig. 16. b, RT-PCR for total RMRP from cell lines expressing control shRNA or Dicer-specific shRNAs. The relative intensity of RMRP is noted at the bottom of the panel. c, RMRP-derived small RNAs are associated with Ago2. Human Ago2 immune complexes were isolated using anti-hAgo2-specific antisera or pre-immune sera, and small RNAs were detected by Northern blotting. Blotting of oligonucleotides (RMRP 20–41 and RMRP AS 41-20) is also shown.