In each panel, representative pseudo-colored images are shown of cytokeratin (upper left), tumor mask (lower left), nuclear (blue) and non-nuclear or membrane compartments (green) (upper right), and target expression (red) after RESA application (lower right). (A) Estrogen receptor, nuclear expression (B) Progesterone receptor, nuclear expression (C) EGFR, membranous expression (D) HER-2, membranous expression (E) HER-3, non-nuclear expression (F) HER-4, non-nuclear expression (G) HER-4, membranous expression (H) HER-4, nuclear expression