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. Author manuscript; available in PMC: 2009 Oct 5.
Published in final edited form as: Oncogene. 2007 Dec 10;27(22):3194–3200. doi: 10.1038/sj.onc.1210979

Figure 4.

Figure 4

Soft agar colony growth of NIH 3T3 cells expressing α and β chains of the IL-3 receptor and P210 BCR-ABL was inhibited by imatinib (IM) or AG490. (a) NIH 3T3 cells expressing IL-3 Rα, IL-3 Rβ and P210 BCR-ABL were seeded into soft agar containing different doses of imatinib (1, 5 and 10 µm). Colonies were stained with crystal blue 3 weeks later. (b) Percentage of agar colony numbers in the presence of imatinib compared to the colony numbers in the absence of imatinib. (c) Bcr-Abl tyrosine phosphorylation following imatinib treatment for 24h; (d) Crystal blue staining agar colonies formed by NIH 3T3 cells expressing the α and β chain of the IL-3 receptor and P210 BCR-ABL in the presence and absence of 10, 20, 50 µm AG490. (e) Percentage of agar colony numbers in AG490-treated cells compared to -untreated cells. Colony counts were assessed on each individual samples twice, P-value is compared with untreated group.