Figure 1.

Binding activity of the phage clones selected by biopanning (A) and of purified scFv (B) to human B7RP-1-Fc using ELISA. We examined the binding of the phage clones (1 × 10¹³ TU/ml) or of the purified scFv fragments (20 µg/ml) toward human B7RP-1-Fc, ICOS-Fc, and other proteins (skim milk, BSA and gelatin), which were coated onto the wells of the immuno plate. Binding phages were detected using the biotinylated anti-M13 mAb and AP-conjugated SA. The scFv fused C-terminally to an E-tag was detected using an anti-E-tag antibody and an AP-conjugated anti-Fc antibody.