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Binding of human-mouse chimeric IgG1 m590, mouse IgG2b 4G11, anti-IGF-IR b subunit antibody GRII and control antibody anti-HIV-1 IgG1 X5 to NWT C43 cells and MCF-7 cells. Ten 10 mg/ml of each antibody were incubated with stably transfected NWT C43 cells or breast cancer cell line MCF-7. Bound chimeric IgG1 m590 and control antibody IgG1 X5 were revealed by PE labeled anti-human IgG, F(ab')2, mouse IgG2b 4G11 and GRII by PE labeled anti-mouse polyclonal antibody.
