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. Author manuscript; available in PMC: 2009 Oct 11.
Published in final edited form as: Biochemistry. 2008 Mar 1;47(12):3917–3925. doi: 10.1021/bi702530z

Figure 2.

Figure 2

Concentration-dependence of arachidonoyltaurine metabolism by pl12-LOX. Maximal rate of metabolism of arachidonoyltaurine (0.78 to 50 μM) by pl12-LOX (closed circles) was determined by monitoring change in absorbance at 236 nm. Due to substrate inhibition, data did not fit hyperbolic Michaelis-Menten equation; vmax/KM was estimated from linear regression (dashed line) at low substrate concentrations. Substrate inhibition was observed at high concentrations of arachidonoyltaurine, but could be partially alleviated by co-incubation with 13-HpODE (open square). All data points represent the average ± SEM of at least two separate trials.