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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9696–9703. doi: 10.1021/bi901283p

FIGURE 4.

FIGURE 4

UV absorption detected size exclusion HPLC profiles of (A) 1,2-cyclohexanedione (1.160 mM, 8 μL, the retention time tR = 11.5 min); and (B) the reaction mixture of DAFP-1 (0.290 mM, 8 μL, tR = 7.1 min) and 1,2-cyclohexanedione (1.160 mM, 8 μL, tR = 11.5 min). The molar ratio of DAFP-1 to 1,2-cyclohexanedione in the reaction mixture was 1:4. The ratio of the area under peak a (Aa = 38.9 mAu · min) to the area under peak a’ (Aa’ = 29.0 mAu · min) is 4:3 indicating only one-fourth of the original amount of 1,2-cyclohexanedione was consumed.