Skip to main content
. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Neuron. 2009 Mar 26;61(6):865–879. doi: 10.1016/j.neuron.2009.02.013

Figure 5. sGCs are required for O2 evoked calcium transients in URX and BAG.

Figure 5

(A–E) Measurements of neural activity by calcium imaging of URX (A–C) and BAG (D,E). Black traces show the average percent change of G-CaMP fluorescence (ΔF/F0) and dark shading shows standard error of the mean (SEM). Concentrations were 21% and 10% O2 (A–C) or 21% and 4% O2 (D,E). (A) URX, gcy-35(ok769) mutants. (B) URX, gcy- 36(db66) mutants. (C) URX, gcy-33(ok232) mutants. (D) BAG, gcy-31(ok296) mutants. (E) BAG, gcy-33(ok232) mutants. Light grey shading indicates the intervals at 21% O2. (F,G) Average ΔF/F0 from 12–22 seconds for indicated genotypes, (F) URX; (G) BAG. The data for wild type animals correspond to Figure 2D,H. Error bars show SEM. Asterisks indicate significance by one-way ANOVA with Dunnett's post-hoc test using wild type in each panel as control groups (***P<0.001, ns not significant). The numbers of recordings analyzed are indicated.