Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966

Color Test for the Measurement of Antibody to T-Strain Mycoplasmas

Robert H Purcell a, D Taylor-Robinson a,1, D Wong a, R M Chanock a
PMCID: PMC276187  PMID: 5941285

Abstract

Purcell, Robert H. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), D. Taylor-Robinson, D. Wong, and R. M. Chanock. Color test for the measurement of antibody to T-strain mycoplasmas. J. Bacteriol. 92:6–12. 1966.—A metabolic inhibition technique for the measurement of antibody to T-strain mycoplasmas was developed, based upon the ability of T-strain mycoplasmas to metabolize urea with the concomitant production of ammonia, and the ability of specific antiserum to inhibit this ammonia production. Phenol red added to the medium served as an indicator of pH change resulting from ammonia production. Specific antiserum to T-strain mycoplasma T-960 was prepared. The T-strain organism was shown to be serologically distinct from the recognized large-colony mycoplasmas. Antibody to mycoplasma strain T-960 in human sera was demonstrated with the metabolic inhibition technique.

Full text

PDF
6

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FORD D. K., RASMUSSEN G., MINKEN J. T-strain pleuropneumonia-like organisms as one cause of non-gonococcal urethritis. Br J Vener Dis. 1962 Mar;38:22–25. doi: 10.1136/sti.38.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ford D. K. CULTURE OF HUMAN GENITAL "T-STRAIN" PLEUROPNEUMONIA-LIKE ORGANISMS. J Bacteriol. 1962 Nov;84(5):1028–1034. doi: 10.1128/jb.84.5.1028-1034.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HAYFLICK L., CHANOCK R. M. MYCOPLASMA SPECIES OF MAN. Bacteriol Rev. 1965 Jun;29:185–221. doi: 10.1128/br.29.2.185-221.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  5. SHEPARD M. C., LUNCEFORD C. D. EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965 Feb;89:265–270. doi: 10.1128/jb.89.2.265-270.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SHEPARD M. C. The recovery of pleuropneumonia-like organisms from Negro men with and without nongonococcal urethritis. Am J Syph Gonorrhea Vener Dis. 1954 Mar;38(2):113–124. [PubMed] [Google Scholar]
  7. SOMERSON N. L., PURCELL R. H., TAYLOR-ROBINSON D., CHANOCK R. M. HEMOLYSIN OF MYCOPLASMA PNEUMONIAE. J Bacteriol. 1965 Mar;89:813–818. doi: 10.1128/jb.89.3.813-818.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. TAYLOR-ROBINSON D., CANCHOLA J., FOX H., CHANOCK R. M. A NEWLY IDENTIFIED ORAL MYCOPLASMA (M. ORALE) AND ITS RELATIONSHIP TO OTHER HUMAN MYCOPLASMAS. Am J Hyg. 1964 Jul;80:135–148. doi: 10.1093/oxfordjournals.aje.a120454. [DOI] [PubMed] [Google Scholar]
  9. TAYLOR-ROBINSON D., LUDWIG W. M., PURCELL R. H., MUFSON M. A., CHANOCK R. M. SIGNIFICANCE OF ANTIBODY TO MYCOPLASMA HOMINIS TYPE 1 AS MEASURED BY INDIRECT HEMAGGLUTINATION. Proc Soc Exp Biol Med. 1965 Apr;118:1073–1083. doi: 10.3181/00379727-118-30049. [DOI] [PubMed] [Google Scholar]
  10. TAYLOR-ROBINSON D., SOMERSON N. L., TURNER H. C., CHANOCK R. M. SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION. J Bacteriol. 1963 Jun;85:1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES