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. 1966 Jul;92(1):35–42. doi: 10.1128/jb.92.1.35-42.1966

Suppression of Fluorophenylalanine Resistance by Mutation to Streptomycin Resistance in Pseudomonas aeruginosa

Judith A Waltho a,1, B W Holloway a
PMCID: PMC276192  PMID: 4957435

Abstract

Waltho, Judith A. (University of Melbourne, Victoria, Australia), and B. W. Holloway. Suppression of fluorophenylalanine resistance by mutation to streptomycin resistance in Pseudomonas aeruginosa. J. Bacteriol. 92:35–42. 1966.—Fluorophenylalanine-resistant mutants (fpa-r) of Pseudomonas aeruginosa have been isolated. By cotransduction analysis, the mutations were shown to have at least two chromosomal locations. One locus (fpaA) showed linkage to three other markers, str, try-3bi, and arg-3, and the order of these four linked markers was found to be try-3bi, arg-3, fpaA, str. The linkage relationships of the other fpa loci are not yet known. The phenotypic expression of resistance at the fpaA locus can be suppressed by mutation of the str locus from str-s to str-r, whereas that at an unlinked fpa locus cannot.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., MARTIN R. G. BIOCHEMICAL ASPECTS OF GENETICS: THE OPERON. Annu Rev Biochem. 1964;33:235–258. doi: 10.1146/annurev.bi.33.070164.001315. [DOI] [PubMed] [Google Scholar]
  2. Anderson W. F., Gorini L., Breckenridge L. Role of ribosomes in streptomycin-activated suppression. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1076–1083. doi: 10.1073/pnas.54.4.1076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COX E. C., WHITE J. R., FLAKS J. G. STREPTOMYCIN ACTION AND THE RIBOSOME. Proc Natl Acad Sci U S A. 1964 Apr;51:703–709. doi: 10.1073/pnas.51.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Couturier M., Desmet L., Thomas R. High pleiotropy of streptomycin mutations in Escherichia coli. Biochem Biophys Res Commun. 1964 Jun 15;16(3):244–248. doi: 10.1016/0006-291x(64)90333-x. [DOI] [PubMed] [Google Scholar]
  5. DAVIES J. E. STUDIES ON THE RIBOSOMES OF STREPTOMYCIN-SENSITIVE AND RESISTANT STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Apr;51:659–664. doi: 10.1073/pnas.51.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIES J., GILBERT W., GORINI L. STREPTOMYCIN, SUPPRESSION, AND THE CODE. Proc Natl Acad Sci U S A. 1964 May;51:883–890. doi: 10.1073/pnas.51.5.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davies J., Anderson P., Davis B. D. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096–1098. doi: 10.1126/science.149.3688.1096. [DOI] [PubMed] [Google Scholar]
  8. Dubnau D., Smith I., Marmur J. Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):724–730. doi: 10.1073/pnas.54.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FARGIE B., HOLLOWAY B. W. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA. Genet Res. 1965 Jul;6:284–299. doi: 10.1017/s0016672300004158. [DOI] [PubMed] [Google Scholar]
  10. FLAKS J. G., COX E. C., WITTING M. L., WHITE J. R. Polypeptide synthesis with ribosomes from streptomycin-resistant and dependent E. coli. Biochem Biophys Res Commun. 1962 May 11;7:390–393. doi: 10.1016/0006-291x(62)90321-2. [DOI] [PubMed] [Google Scholar]
  11. FRIEDMAN S. M., WEINSTEIN I. B. LACK OF FIDELITY IN THE TRANSLATION OF SYNTHETIC POLYRIBONUCLEOTIDES. Proc Natl Acad Sci U S A. 1964 Oct;52:988–996. doi: 10.1073/pnas.52.4.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GORINI L., KATAJA E. PHENOTYPIC REPAIR BY STREPTOMYCIN OF DEFECTIVE GENOTYPES IN E. COLI. Proc Natl Acad Sci U S A. 1964 Mar;51:487–493. doi: 10.1073/pnas.51.3.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOLLOWAY B. W., EGAN J. B., MONK M. Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1960 Aug;38:321–329. doi: 10.1038/icb.1960.34. [DOI] [PubMed] [Google Scholar]
  14. HOLLOWAY B. W. Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol. 1955 Dec;13(3):572–581. doi: 10.1099/00221287-13-3-572. [DOI] [PubMed] [Google Scholar]
  15. HOLLOWAY B. W., HODGINS L., FARGIE B. UNLINKED LOCI AFFECTING RELATED BIOSYNTHETIC STEPS IN PSEUDOMONAS AERUGINOSA. Nature. 1963 Aug 31;199:926–927. doi: 10.1038/199926a0. [DOI] [PubMed] [Google Scholar]
  16. HOLLOWAY B. W., MONK M., HODGINS L., FARGIE B. Effects of radiation on transduction on Pseudomonas aeruginosa. Virology. 1962 Sep;18:89–94. doi: 10.1016/0042-6822(62)90180-0. [DOI] [PubMed] [Google Scholar]
  17. Kaji H., Kaji A. Specific binding of sRNA to ribosomes: effect of streptomycin. Proc Natl Acad Sci U S A. 1965 Jul;54(1):213–219. doi: 10.1073/pnas.54.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LEDERBERG E. M., CAVALLI-SFORZA L., LEDERBERG J. INTERACTION OF STREPTOMYCIN AND A SUPPRESSOR FOR GALACTOSE FERMENTATION IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Apr;51:678–682. doi: 10.1073/pnas.51.4.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. MAAS W. K. Studies on repression of arginine biosynthesis in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:183–191. doi: 10.1101/sqb.1961.026.01.023. [DOI] [PubMed] [Google Scholar]
  21. MAGER J., BENEDICT M., ARTMAN M. A common site of action of polyamines and streptomycin. Biochim Biophys Acta. 1962 Jul 30;62:202–204. doi: 10.1016/0006-3002(62)90519-x. [DOI] [PubMed] [Google Scholar]
  22. SPEYER J. F., LENGYEL P., BASILIO C. Ribosomal localization of streptomycin sensitivity. Proc Natl Acad Sci U S A. 1962 Apr 15;48:684–686. doi: 10.1073/pnas.48.4.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SZER W., OCHOA S. COMPLEXING ABILITY AND CODING PROPERTIES OF SYNTHETIC POLYNUCLEOTIDES. J Mol Biol. 1964 Jun;8:823–834. doi: 10.1016/s0022-2836(64)80163-7. [DOI] [PubMed] [Google Scholar]
  24. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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