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. 1966 Jul;92(1):63–71. doi: 10.1128/jb.92.1.63-71.1966

Nutrition and Metabolism of Marine Bacteria XV. Relation of Na+-Activated Transport to the Na+ Requirement of a Marine Pseudomonad for Growth1

Gabriel R Drapeau a,2, Tibor I Matula a, Robert A MacLeod a
PMCID: PMC276196  PMID: 5941284

Abstract

Drapeau, Gabriel R., (McGill University, Montreal, Quebec, Canada), Tibor I. Matula, and Robert A. MacLeod. Nutrition and metabolism of marine bacteria. XV. Relation of Na+-activated transport to the Na+ requirement of a marine pseudomonad for growth. J. Bacteriol. 92:63–71. 1966.—A marine pseudomonad was found to require 50 to 100 mm Na+ for maximal rate of oxidation of d-galactose and for the transport of d-fucose-H3 into the cells. The same organism required 150 to 200 mm Na+ for the oxidation of l-alanine and for the transport of φ-aminoisobutyric acid-C14 (AIB-C14) into the cells. Competition studies indicated that d-galactose and d-fucose on the one hand and l-alanine and AIB on the other shared common carriers for transporting the compounds into the cells. This parallelism in Na+ response for oxidation and transport extended to growth when l-alanine was the sole carbon source in the medium. When d-galactose was the sole carbon source, an amount of Na+ equal to that with l-alanine was needed. KCN and dinitrophenol but not ouabain inhibited the uptake of AIB-C14 by the cells. K+ in addition to Na+ was required for transport, and both Mg++ and either Cl− or Br− were stimulatory. Photobacterium fischeri was also found to require Na+ specifically for the uptake of AIB-C14 by the cells.

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Selected References

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  1. BROCK T. D., MOO-PENN G. An amino acid transport system in Streptococcus faecium. Arch Biochem Biophys. 1962 Aug;98:183–190. doi: 10.1016/0003-9861(62)90171-6. [DOI] [PubMed] [Google Scholar]
  2. Crane R. K. Na+ -dependent transport in the intestine and other animal tissues. Fed Proc. 1965 Sep-Oct;24(5):1000–1006. [PubMed] [Google Scholar]
  3. DRAPEAU G. R., MACLEOD R. A. NUTRITION AND METABOLISM OF MARINE BACTERIA. XII. ION ACTIVATION OF ADENOSINE TRIPHOSPHATASE IN MEMBRANES OF MARINE BACTERIAL CELLS. J Bacteriol. 1963 Jun;85:1413–1419. doi: 10.1128/jb.85.6.1413-1419.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drapeau G. R., MacLeod R. A. A role for inorganic ions in the maintenance of intracellular solute concentrations in a marine pseudomonad. Nature. 1965 May 1;206(983):531–531. doi: 10.1038/206531a0. [DOI] [PubMed] [Google Scholar]
  5. FOX M., THIER S., ROSENBERG L., SEGAL S. IONIC REQUIREMENTS FOR AMINO ACID TRANSPORT IN THE RAT KIDNEY CORTEX SLICE. I. INFLUENCE OF EXTRACELLULAR IONS. Biochim Biophys Acta. 1964 Jan 27;79:167–176. doi: 10.1016/0926-6577(64)90049-x. [DOI] [PubMed] [Google Scholar]
  6. HOFFEE P., ENGLESBERG E. Effect of metabolic activity on the glucose permease of bacterial cells. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1759–1765. doi: 10.1073/pnas.48.10.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MACLEOD R. A., CLARIDGE C. A., HORI A., MURRAY J. F. Observations on the function of sodium in the metabolism of a marine bacterium. J Biol Chem. 1958 Jun;232(2):829–834. [PubMed] [Google Scholar]
  8. MACLEOD R. A., HORI A. Nutrition and metabolism of marine bacteria. VIII. Tricarboxylic acid cycle enzymes in a marine bacterium and their response to inorganic salts. J Bacteriol. 1960 Oct;80:464–471. doi: 10.1128/jb.80.4.464-471.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MACLEOD R. A., ONOFREY E. Nutrition and metabolism of marine bacteria. III. The relation of sodium and potassium to growth. J Cell Physiol. 1957 Dec;50(3):389–401. doi: 10.1002/jcp.1030500305. [DOI] [PubMed] [Google Scholar]
  10. MACLEOD R. A., ONOFREY E. Nutrition and metabolism of marine bacteria. VI. Quantitative requirements for halides, magnesium, calcium, and iron. Can J Microbiol. 1957 Aug;3(5):753–759. doi: 10.1139/m57-085. [DOI] [PubMed] [Google Scholar]
  11. MACLEOD R. A. THE QUESTION OF THE EXISTENCE OF SPECIFIC MARINE BACTERIA. Bacteriol Rev. 1965 Mar;29:9–24. [PMC free article] [PubMed] [Google Scholar]
  12. POST R. L., MERRITT C. R., KINSOLVING C. R., ALBRIGHT C. D. Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J Biol Chem. 1960 Jun;235:1796–1802. [PubMed] [Google Scholar]
  13. PRATT D., HAPPOLD F. C. Requirements for indole production by cells and extracts of a marine bacterium. J Bacteriol. 1960 Aug;80:232–236. doi: 10.1128/jb.80.2.232-236.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RHODES M. E., PAYNE W. J. Further observations on effects of cations on enzyme induction in marine bacteria. Antonie Van Leeuwenhoek. 1962;28:302–314. doi: 10.1007/BF02538743. [DOI] [PubMed] [Google Scholar]
  15. SCHULTZ S. G., ZALUSKY R. INTERACTIONS BETWEEN ACTIVE SODIUM TRANSPORT AND ACTIVE AMINO-ACID TRANSPORT IN ISOLATED RABBIT ILEUM. Nature. 1965 Jan 16;205:292–294. doi: 10.1038/205292a0. [DOI] [PubMed] [Google Scholar]
  16. TAKACS F. P., MATULA T. I., MACLEOD R. A. NUTRITION AND METABOLISM OF MARINE BACTERIA. XIII. INTRACELLULAR CONCENTRATIONS OF SODIUM AND POTASSIUM IONS IN A MARINE PSEUDOMONAD. J Bacteriol. 1964 Mar;87:510–518. doi: 10.1128/jb.87.3.510-518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TOMLINSON N., MACLEOD R. A. Nutrition and metabolism of marine bacteria. IV. The participation of Na+, K+, and Mg++ salts in the oxidation of exogenous substrates by a marine bacterium. Can J Microbiol. 1957 Jun;3(4):627–638. doi: 10.1139/m57-068. [DOI] [PubMed] [Google Scholar]

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