Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jul;92(1):102–106. doi: 10.1128/jb.92.1.102-106.1966

Density Gradient Centrifugation Studies on Rabies Virus

A Robert Neurath a,1, Tadeusz J Wiktor a, Hilary Koprowski a
PMCID: PMC276202  PMID: 5941269

Abstract

Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102–106. 1966.—Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained by rate zonal centrifugation in preformed sucrose gradients, it was possible to calculate a sedimentation coefficient of about 600S for rabies virus. Sedimentation coefficients of about 23S and 10S were calculated for two soluble rabies antigens present in infected tissue-culture fluids, and they showed a density of 1.26 g/ml in cesium chloride solutions.

Full text

PDF
102

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRAWFORD L. V. A study of the Rous sarcoma virus by density gradient centrifugation. Virology. 1960 Oct;12:143–153. doi: 10.1016/0042-6822(60)90190-2. [DOI] [PubMed] [Google Scholar]
  2. GOODHEART C. R. A DENSITY MUTANT OF ENCEPHALOMYOCARDITIS VIRUS. Virology. 1965 Jul;26:466–477. doi: 10.1016/0042-6822(65)90009-7. [DOI] [PubMed] [Google Scholar]
  3. GRASSET N., ATANASIU P. [Study of the diffusion in agar of fixed rabies virus antigens obtained in tissue cultures]. Ann Inst Pasteur (Paris) 1961 Nov;101:639–647. [PubMed] [Google Scholar]
  4. HAMPARIAN V. V., HILLEMAN M. R., KETLER A. Contributions to characterization and classification of animal viruses. Proc Soc Exp Biol Med. 1963 Apr;112:1040–1050. doi: 10.3181/00379727-112-28247. [DOI] [PubMed] [Google Scholar]
  5. Kaplan M. M., Wiktor T., Koprowski H. An intracerebral assay procedure in mice for chemical inactivation of rabies virus. Bull World Health Organ. 1966;34(2):293–297. [PMC free article] [PubMed] [Google Scholar]
  6. Kilkson R. Structure and evolution of viruses, a limiting theory. Exp Cell Res. 1965 Aug;39(1):265–285. doi: 10.1016/0014-4827(65)90029-7. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  9. MEAD T. H. Purification of rabies soluble antigens. J Gen Microbiol. 1962 Mar;27:397–414. doi: 10.1099/00221287-27-3-397. [DOI] [PubMed] [Google Scholar]
  10. MEAD T. H. The characterization of rabies soluble antigens. J Gen Microbiol. 1962 Mar;27:415–426. doi: 10.1099/00221287-27-3-415. [DOI] [PubMed] [Google Scholar]
  11. Neurath A. R. Fluorometric estimation of antigens (antibodies). Z Naturforsch B. 1965 Oct;20(10):974–976. doi: 10.1515/znb-1965-1008. [DOI] [PubMed] [Google Scholar]
  12. PFAU C. J. BIOPHYSICAL AND BIOCHEMICAL CHARACTERIZATION OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS. 1. DENSITY GRADIENT STUDIES. Acta Pathol Microbiol Scand. 1965;63:188–197. doi: 10.1111/apm.1965.63.2.188. [DOI] [PubMed] [Google Scholar]
  13. POLSON A., WESSELS P. Particle size of soluble antigen of rabies virus. Proc Soc Exp Biol Med. 1953 Nov;84(2):317–320. doi: 10.3181/00379727-84-20632. [DOI] [PubMed] [Google Scholar]
  14. ROIZMAN B., ROANE P. R., Jr A physical difference between two strains of herpes simplex virus apparent on sedimentation in cesium chloride. Virology. 1961 Sep;15:75–79. doi: 10.1016/0042-6822(61)90079-4. [DOI] [PubMed] [Google Scholar]
  15. STENBACK W. A., DURAND D. P. HOST INFLUENCE ON THE DENSITY OF NEWCASTLE DISEASE VIRUS (NDV). Virology. 1963 Aug;20:545–551. doi: 10.1016/0042-6822(63)90278-2. [DOI] [PubMed] [Google Scholar]
  16. VINOGRAD J., HEARST J. E. Equilibrium sedimentation of macromolecules and viruses in a density gradient. Fortschr Chem Org Naturst. 1962;20:373–422. [PubMed] [Google Scholar]
  17. WIKTOR T. J., FERNANDES M. V., KOPROWSKI H. CULTIVATION OF RABIES VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38. J Immunol. 1964 Sep;93:353–366. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES