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. 1966 Jul;92(1):144–147. doi: 10.1128/jb.92.1.144-147.1966

Ultraviolet Mutagenesis in Bacteriophage T4 II. Photoreversal of Mutational Lesions

John W Drake 1
PMCID: PMC276208  PMID: 5941273

Abstract

Drake, John W. (University of Illinois, Urbana). Ultraviolet mutagenesis in bacteriophage T4. II. Photoreversal of mutational lesions. J. Bacteriol. 92:144–147. 1966.—T4r mutations were induced by ultraviolet irradiation of extracellular phage particles, using a phage mutant, v, which is particularly susceptible to photoreactivation. Most of the induced r mutations could be subsequently photoreversed intracellularly with white light. Ultraviolet irradiation induces both transitions and sign mutations, and both were susceptible to photoreversal. The results suggest that two very different types of mutational lesions may arise from a common type of photochemical lesion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. GENETIC CONTROL OF DNA BREAKDOWN AND REPAIR IN E. COLI K-12 TREATED WITH MITOMYCIN C OR ULTRAVIOLET LIGHT. Z Vererbungsl. 1964 Dec 30;95:345–350. doi: 10.1007/BF01268667. [DOI] [PubMed] [Google Scholar]
  2. CRICK F. H., BARNETT L., BRENNER S., WATTS-TOBIN R. J. General nature of the genetic code for proteins. Nature. 1961 Dec 30;192:1227–1232. doi: 10.1038/1921227a0. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R. Experiments on photoreactivation of bacteriophages inactivated with ultraviolet radiation. J Bacteriol. 1950 Mar;59(3):329–347. doi: 10.1128/jb.59.3.329-347.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drake J. W. Heteroduplex heterozygotes in bacteriophage T4 involving mutations of various dimensions. Proc Natl Acad Sci U S A. 1966 Mar;55(3):506–512. doi: 10.1073/pnas.55.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Drake J. W. Ultraviolet mutagenesis in bacteriophage T-4. I. Irradiation of extracellular phage particles. J Bacteriol. 1966 May;91(5):1775–1780. doi: 10.1128/jb.91.5.1775-1780.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ELLISON S. A., FEINER R. R., HILL R. F. A host effect on bacteriophage survival after ultraviolet irradiation. Virology. 1960 May;11:294–296. doi: 10.1016/0042-6822(60)90069-6. [DOI] [PubMed] [Google Scholar]
  7. FREESE E. On the molecular explanation of spontaneous and induced mutations. Brookhaven Symp Biol. 1959 Nov;NO:63–75. [PubMed] [Google Scholar]
  8. HARM W. Mutants of phage T4 with increased sensitivity to ultraviolet. Virology. 1963 Jan;19:66–71. doi: 10.1016/0042-6822(63)90025-4. [DOI] [PubMed] [Google Scholar]
  9. HOWARD B. D., TESSMAN I. IDENTIFICATION OF THE ALTERED BASES IN MUTATED SINGLE-STRANDED DNA. 3. MUTAGENESIS BY ULTRAVIOLET LIGHT. J Mol Biol. 1964 Aug;9:372–375. doi: 10.1016/s0022-2836(64)80214-x. [DOI] [PubMed] [Google Scholar]
  10. KAPLAN R. W., WINKLER U., WOLF-ELLMAUER H. Induction and reversion of c-mutations by irradiation of the extracellular k-phage of Serratia. Nature. 1960 Apr 23;186:330–331. doi: 10.1038/186330b0. [DOI] [PubMed] [Google Scholar]
  11. LERMAN L. S. The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963 Jan 15;49:94–102. doi: 10.1073/pnas.49.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MATNEY T. S., SHANKEL D. M., WYSS O. Mutations induced by ultraviolet light without attendant lethality. J Bacteriol. 1958 Feb;75(2):180–183. doi: 10.1128/jb.75.2.180-183.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NEWCOMBE H. B., WHITEHEAD H. A. Photoreversal of ultraviolet-induced mutagenic and lethal effects in Escherichia coli. J Bacteriol. 1951 Mar;61(3):243–251. doi: 10.1128/jb.61.3.243-251.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NOVICK A., SZILARD L. Experiments on light-reactivation of ultra-violet inactivated bacteria. Proc Natl Acad Sci U S A. 1949 Oct;35(10):591–600. doi: 10.1073/pnas.35.10.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ONO J., WILSON R. G., GROSSMAN L. EFFECTS OF ULTRAVIOLET LIGHT ON THE TEMPLATE PROPERTIES OF POLYCYTIDYLIC ACID. J Mol Biol. 1965 Mar;11:600–612. doi: 10.1016/s0022-2836(65)80014-6. [DOI] [PubMed] [Google Scholar]
  16. RUPERT C. S., GOODGAL S. H., HERRIOTT R. M. Photoreactivation in vitro of ultraviolet-inactivated Hemophilus influenzae transforming factor. J Gen Physiol. 1958 Jan 20;41(3):451–471. doi: 10.1085/jgp.41.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Setlow J. K., Boling M. E., Bollum F. J. The chemical nature of photoreactivable lesions in DNA. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1430–1436. doi: 10.1073/pnas.53.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Setlow R. B., Carrier W. L., Bollum F. J. Pyrimidine dimers in UV-irradiated poly dI:dC. Proc Natl Acad Sci U S A. 1965 May;53(5):1111–1118. doi: 10.1073/pnas.53.5.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WITKIN E. M. PHOTOREVERSAL AND "DARK REPAIR" OF MUTATIONS TO PROTOTROPHY INDUCED BY ULTRAVIOLET LIGHT IN PHOTOREACTIVABLE AND NON-PHOTOREACTIVABLE STRAINS OF ESCHERICHIA COLI. Mutat Res. 1964 May;106:22–36. doi: 10.1016/0027-5107(64)90049-1. [DOI] [PubMed] [Google Scholar]
  20. ZELLE M. R., OGG J. E., HOLLAENDER A. Photoreactivation of induced mutation and inactivation of Escherichia coli exposed to various wave lengths of monochromatic ultraviolet radiation. J Bacteriol. 1958 Feb;75(2):190–198. doi: 10.1128/jb.75.2.190-198.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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