Abstract
Duc-Nguyen, Huu (The Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Replication of mumps virus in human leukocyte cultures. J. Bacteriol. 92:258–265. 1966.—Human peripheral leukocyte cultures maintained in the presence of phytohemagglutinin (PHA) were found to support to some extent the replication of mumps virus. When such cultures were exposed, within 24 hr after their initiation, to a high input multiplicity of virus, successful infection, as determined by immunofluorescence and plaque assays, did not become evident before the 3rd or 4th day. On exposure of cultures 4 to 5 days old, viral replication was detectable within 2 days. In both instances, peak immunofluorescence and virus titers were reached when the cultures were 7 to 9 days old and composed mainly of blast forms. With decreasing input multiplicities of infection, cells containing viral antigen and production of infectious viral progeny became detectable with increasing delay. No significant viral replication was noted in surviving cells maintained in the absence of PHA. These results indicate that mainly, if not solely, the PHA-stimulated cells of the lymphocytic series support viral multiplication. The extent of the infectious process was limited, however, because the life span of the cultures was not significantly shortened, the yields of infectious virus per immunofluorescent cell were at all times low, and most infected cells contained only a few well-delineated small masses of antigen, suggestive of an abortive infection. Only fresh cultures were capable of synthesizing interferon on stimulation by mumps, Newcastle disease, or Sendai viruses. When the cultures were set up in the presence of PHA, this capacity was lost within 24 hr. PHA per sefailed to induce detectable production of an interferon under the conditions used. The implications of these findings are discussed.
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- BERG R. B. Multiplication of ECHO 9 virus in suspensions of human leucocytes. Proc Soc Exp Biol Med. 1961 Dec;108:742–744. [PubMed] [Google Scholar]
- CANTELL K., PAUCKER K. QUANTITATIVE STUDIES ON VIRAL INTERFERENCE IN SUSPENDED L CELLS. IV. PRODUCTION AND ASSAY OF INTERFERON. Virology. 1963 Sep;21:11–21. doi: 10.1016/0042-6822(63)90298-8. [DOI] [PubMed] [Google Scholar]
- GRESSER I., CHANY C. MULTIPLICATION OF POLIOVIRUS TYPE I IN PREPARATIONS OF HUMAN LEUKOCYTES AND ITS INHIBITION BY INTERFERON. J Immunol. 1964 Jun;92:889–895. [PubMed] [Google Scholar]
- GRESSER I. Production of interferon by suspensions of human leucocytes. Proc Soc Exp Biol Med. 1961 Dec;108:799–803. doi: 10.3181/00379727-108-27072. [DOI] [PubMed] [Google Scholar]
- HENLE G., DEINHARDT F., BERGS V. V., HENLE W. Studies on persistent infections of tissue cultures. I. General aspects of the system. J Exp Med. 1958 Oct 1;108(4):537–560. doi: 10.1084/jem.108.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JAHMIAS A. J., KIBRICK S., ROSAN R. C. VIRAL LEUKOCYTE INTERRELATIONSHIPS. I. MULTIPLICATION OF A DNA VIRUS--HERPES SIMPLEX--IN HUMAN LEUKOCYTE CULTURES. J Immunol. 1964 Jul;93:69–74. [PubMed] [Google Scholar]
- LEE S. H., OZERE R. L. PRODUCTION OF INTERFERON BY HUMAN MONONUCLEAR LEUCOCYTES. Proc Soc Exp Biol Med. 1965 Jan;118:190–195. doi: 10.3181/00379727-118-29794. [DOI] [PubMed] [Google Scholar]
- MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
- NOWELL P. C. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960 May;20:462–466. [PubMed] [Google Scholar]
- PAUCKER K. THE SEROLOGIC SPECIFICITY OF INTERFERON. J Immunol. 1965 Mar;94:371–378. [PubMed] [Google Scholar]
- RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
- WALKER D. L., HINZE H. C. A carrier state of mumps virus in human conjunctiva cells. I. General characteristics. J Exp Med. 1962 Nov 1;116:739–750. doi: 10.1084/jem.116.5.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wheelock E. F. Interferon-like virus-inhibitor induced in human leukocytes by phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. [PubMed] [Google Scholar]