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. 1966 Sep;92(3):639–644. doi: 10.1128/jb.92.3.639-644.1966

Isotopic Method for Estimating Microbial Cell Volumes1

Della F Reid a, Hilmer A Frank b
PMCID: PMC276303  PMID: 4958775

Abstract

Reid, Della F. (University of Hawaii, Honolulu), and Hilmer A. Frank. Isotopic method for estimating microbial cell volumes. J. Bacteriol. 92:639–644. 1966.—A sensitive isotopic method was developed for estimating the mean cell volume in small numbers of bacterial spores by measuring directly the extracellular fluid volume when cells are present in a saturated inulin solution containing inulin-C14 at low specific activity. The method minimizes errors due to solute adsorption to the cells and avoids errors arising from differences in cell packing. The effects of these sources of error on cell volume and intercellular space estimations made with nonpenetrating solutes are discussed.

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Selected References

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  1. BRECHER G., JAKOBEK E. F., SCHNEIDERMAN M. A., WILLIAMS G. Z., SCHMIDT P. J. Size distribution of erythrocytes. Ann N Y Acad Sci. 1962 Jun 29;99:242–261. doi: 10.1111/j.1749-6632.1962.tb45310.x. [DOI] [PubMed] [Google Scholar]
  2. BRITT E. M., GERHARDT P. Bacterial permeability; total uptake of lysine by intact cells, protoplasts, and cell walls of Micrococcus lysodeikticus. J Bacteriol. 1958 Sep;76(3):288–293. doi: 10.1128/jb.76.3.288-293.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHURCH B. D., HALVORSON H., HALVORSON H. O. Studies on spore germination: its independence from alanine racemase activity. J Bacteriol. 1954 Oct;68(4):393–399. doi: 10.1128/jb.68.4.393-399.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CIRILLO V. P. Mechanism of glucose transport across the yeast cell membrane. J Bacteriol. 1962 Sep;84:485–491. doi: 10.1128/jb.84.3.485-491.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CONWAY E. J., DOWNEY M. An outer metabolic region of the yeast cell. Biochem J. 1950 Sep;47(3):347–355. doi: 10.1042/bj0470347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HILL G. A., FUNG J., MARCUS S. Polyvinylpyrrolidone as an indicator for measuring intercellular space in packed-cell pellets. J Bacteriol. 1962 Jul;84:191–192. doi: 10.1128/jb.84.1.191-192.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hurwitz C., Braun C. B., Peabody R. A. Washing bacteria by centrifugation through a water-immiscible layer of silicones. J Bacteriol. 1965 Dec;90(6):1692–1695. doi: 10.1128/jb.90.6.1692-1695.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KUBITSCHEK H. E. Electronic counting and sizing of bacteria. Nature. 1958 Jul 26;182(4630):234–235. doi: 10.1038/182234a0. [DOI] [PubMed] [Google Scholar]
  9. KULKA R. G. Colorimetric estimation of ketopentoses and ketohexoses. Biochem J. 1956 Aug;63(4):542–548. doi: 10.1042/bj0630542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MACDONALD R. E., GERHARDT P. Bacterial permeability: the uptake and oxidation of citrate by Escherichia coli. Can J Microbiol. 1958 Apr;4(2):109–124. doi: 10.1139/m58-013. [DOI] [PubMed] [Google Scholar]
  11. MITCHELL P., MOYLE J. Permeability of the envelopes of Staphylococcus aureus to some salts, amino acids, and non-electrolytes. J Gen Microbiol. 1959 Apr;20(2):434–441. doi: 10.1099/00221287-20-2-434. [DOI] [PubMed] [Google Scholar]
  12. MIZUSHIMA S., MACHIDA Y., KITAHARA K. QUANTITATIVE STUDIES ON GLYCOLYTIC ENZYMES IN LACTOBACILLUS PLANTARUM. I. CONCENTRATION OF INORGANIC IONS AND COENZYMES IN FERMENTING CELLS. J Bacteriol. 1963 Dec;86:1295–1300. doi: 10.1128/jb.86.6.1295-1300.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PHELPS C. F. THE PHYSICAL PROPERTIES OF INULIN SOLUTIONS. Biochem J. 1965 Apr;95:41–47. doi: 10.1042/bj0950041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RECORD B. R., TAYLOR R., MILLER D. S. The survival of Escherichia coli on drying and rehydration. J Gen Microbiol. 1962 Sep;28:585–598. doi: 10.1099/00221287-28-4-585. [DOI] [PubMed] [Google Scholar]
  15. ROTHSTEIN A. Role of the cell membrane in the metabolism of inorganic electrolytes by microorganisms. Bacteriol Rev. 1959 Dec;23(4):175–201. doi: 10.1128/br.23.4.175-201.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ZIMMERMAN L. Survival of Serratia marcescens after freeze-drying or aerosolization at unfavorable humidity. I. Effects of sugars. J Bacteriol. 1962 Dec;84:1297–1302. doi: 10.1128/jb.84.6.1297-1302.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]

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