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. 1966 Sep;92(3):662–667. doi: 10.1128/jb.92.3.662-667.1966

Binding of Homologous Polymerized Deoxyribonucleic Acid by Streptomyces griseus

G R Germaine 1, D L Anderson 1
PMCID: PMC276306  PMID: 5922540

Abstract

Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662–667. 1966—An irreversible P32 deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus cytosine (GC) S. griseus DNA was bound by late exponential –early stationary phase cultures. Simultaneous addition of deoxyribonuclease with the P32-DNA to maximally receptive cultures reduced the uptake by 98%. Saturation of the binding ability occurred after 20-min incubation of cells with P32-DNA. Preparative cesium chloride density gradient centrifugation of the P32-DNA revealed that 98% of the label banded in the position expected for S. griseus DNA. Sedimentation of the P32-DNA at both 23 and 37 μg/ml indicated a molecular weight of 10.8 million. Attempts to transform S. griseus S104 auxotrophs to prototrophy by use of immediate and delayed selective procedures were unsuccessful.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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