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. 1966 Oct;92(4):820–827. doi: 10.1128/jb.92.4.820-827.1966

Immunological Tolerance to Microbial Antigens II. Suppressed Antibody Plaque Formation to Shigella Antigen by Spleen Cells from Tolerant Mice

Herman Friedman 1
PMCID: PMC276340  PMID: 5926751

Abstract

Friedman, Herman (Albert Einstein Medical Center, Philadelphia, Pa.). Immunological tolerance to microbial antigens. II. Suppressed antibody plaque formation to Shigella antigen by spleen cells from tolerant mice. J. Bacteriol. 92:820–827. 1966.—An indirect, localized, antibody plaque procedure has been used to demonstrate a marked difference in the number of antibody plaques formed with spleen cell suspensions from normal and Shigella-tolerant mice. Whereas challenge with soluble Shigella antigen (SSA) into normal mice, ranging in age from 4 to 40 weeks, resulted in a rapid rise in antibody plaque formation to Shigella-treated sheep erythrocytes, there was only a slight increase in plaque formation with spleen cell suspensions from similarly challenged mice which had been made tolerant to Shigella antigen during neonatal life. Apparently, the suppression of plaque formation to Shigella in tolerant animals was specific, since both Shigella-tolerant and normal mice responded equally well to untreated sheep red blood cells after challenge with sheep erythrocytes only. Spleen cells from nonchallenged Shigella-tolerant mice did not form significant numbers of antibody plaques to SSA-treated red blood cells during an observation period of 4 to 30 weeks after neonatal administration of antigen. “Nonspecific” increases in plaque formation to untreated sheep red cells occurred with spleen cell suspensions from both normal and SSA-tolerant mice after challenge injection with Shigella antigen, with or without sheep erythrocytes. Such a response suggested an adjuvant effect for the endotoxin-containing Shigella antigen even in mice tolerant to the agglutinogenic moiety of SSA. The results of these experiments support the view that specific antibody-forming cells are either absent or in low number in lymphoid tissue from mice specifically tolerant to Shigella antigens. It seems unlikely that the low postchallenge agglutinin titers of tolerant mice are due to suppressed antibody formation by normal numbers of individual antibody-producing cells, or due to “masking” of normal antibody production by persisting circulating antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARGYRIS B. F. Adoptive tolerance; transfer of the tolerant state. J Immunol. 1963 Jan;90:29–34. [PubMed] [Google Scholar]
  2. BATTISTO J. R., CHASE M. W. IMMUNOLOGICAL UNRESPONSIVENESS TO SENSITIZATION WITH SIMPLE CHEMICAL COMPOUNDS; A SEARCH FOR ANTIBODY-ABSORBING DEPOTS OF ALLERGEN. J Exp Med. 1963 Dec 1;118:1021–1035. doi: 10.1084/jem.118.6.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
  4. FARBER M. B., HARRIS S., HARRIS T. N., OGBURN C. A. Studies on the transfer of lymph node cells. VII. Transfer of cells incubated in vitro with filtrates of trypsin-treated suspensions of Shigella paradysenteriae. J Exp Med. 1956 Nov 1;104(5):645–661. doi: 10.1084/jem.104.5.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRIEDMAN H. ABSENCE OF ANTIBODY PLAQUE FORMING CELLS IN SPLEENS OF THYMECTOMIZED MICE IMMUNIZED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1965 Apr;118:1176–1180. doi: 10.3181/00379727-118-30073. [DOI] [PubMed] [Google Scholar]
  6. FRIEDMAN H. ADOPTIVE TOLERANCE TO SHIGELLA ANTIGEN IN IRRADIATED MICE RECEIVING SPLEEN CELL TRANSPLANTS FROM UNRESPONSIVE DONORS. J Immunol. 1965 Mar;94:352–357. [PubMed] [Google Scholar]
  7. FRIEDMAN H. DISTRIBUTION OF ANTIBODY PLAQUE FORMING CELLS IN VARIOUS TISSUES OF SEVERAL STRAINS OF MICE INJECTED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1964 Nov;117:526–530. doi: 10.3181/00379727-117-29628. [DOI] [PubMed] [Google Scholar]
  8. FRIEDMAN H. FAILURE OF SPLEEN CELLS FROM IMMUNOLOGICALLY TOLERANT MICE TO FORM ANTIBODY PLAQUES TO SHEEP ERYTHROCYTES IN AGAR GEL. Nature. 1965 Jan 30;205:508–509. doi: 10.1038/205508b0. [DOI] [PubMed] [Google Scholar]
  9. FRIEDMAN H., GABY W. L. Immunologic unresponsiveness in mice following neonatal exposure to Shigella antigens. J Immunol. 1960 Nov;85:478–482. [PubMed] [Google Scholar]
  10. FRIEDMAN H. Transfer of antibody formation by spleen cells from immunologically unresponsive mice. J Immunol. 1962 Aug;89:257–263. [PubMed] [Google Scholar]
  11. Friedman H. Immunological tolerance to microbial antigens I. Absence of specific antibody-containing cells in lymphoid tissue of mice injected at birth with Shigella soluble antigen. J Bacteriol. 1966 Aug;92(2):390–397. doi: 10.1128/jb.92.2.390-397.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Friedman H. Monospecific antibody plaque formation by spleen cells from mice immunized with sheep and chicken erythrocytes. Experientia. 1964 Oct 15;20(10):564–565. doi: 10.1007/BF02150293. [DOI] [PubMed] [Google Scholar]
  13. INGRAHAM J. S., BUSSARD A. APPLICATION OF A LOCALIZED HEMOLYSIN REACTION FOR SPECIFIC DETECTION OF INDIVIDUAL ANTIBODY-FORMING CELLS. J Exp Med. 1964 Apr 1;119:667–684. doi: 10.1084/jem.119.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  15. LANDY M., SANDERSON R. P., BERNSTEIN M. T., LERNER E. M., 2nd INVOLVEMENT OF THYMUS IN IMMUNE RESPONSE OF RABBITS TO SOMATIC POLYSACCHARIDES OF GRAM-NEGATIVE BACTERIA. Science. 1965 Mar 26;147(3665):1591–1592. doi: 10.1126/science.147.3665.1591. [DOI] [PubMed] [Google Scholar]
  16. MITCHISON N. A. Tolerance of erythrocytes in poultry: loss and abolition. Immunology. 1962 May;5:359–369. [PMC free article] [PubMed] [Google Scholar]
  17. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NOSSAL G. J. The induction of immunological tolerance in rats to foreign erythrocytes. Aust J Exp Biol Med Sci. 1958 Jun;36(3):235–244. doi: 10.1038/icb.1958.25. [DOI] [PubMed] [Google Scholar]
  19. RICHARDSON M., DUTTON R. W. ANTIBODY SYNTHESIZING CELLS: APPEARANCE AFTER SECONDARY ANTIGENIC STIMULATION IN VITRO. Science. 1964 Oct 30;146(3644):655–656. doi: 10.1126/science.146.3644.655. [DOI] [PubMed] [Google Scholar]
  20. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROWLEY D. A., FITCH F. W. THE MECHANISM OF TOLERANCE PRODUCED IN RATS TO SHEEP ERYTHROCYTES. II. THE PLAQUE-FORMING CELL AND ANTIBODY RESPONSE TO MULTIPLE INJECTIONS OF ANTIGEN BEGUN AT BIRTH. J Exp Med. 1965 May 1;121:683–695. doi: 10.1084/jem.121.5.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SERCARZ E. E., COONS A. H. THE ABSENCE OF ANTIBODY-PRODUCING CELLS DURING UNRESPONSIVENESS TO BSA IN THE MOUSE. J Immunol. 1963 Mar;90:478–491. [PubMed] [Google Scholar]
  23. STASTNY P. PERSISTENCE OF ACQUIRED TOLERANCE IN CELLS TRANSFERRED TO AN ANTIGEN-FREE ENVIRONMENT. J Immunol. 1964 Apr;92:626–629. [PubMed] [Google Scholar]
  24. STELOS P., TALMAGE D. W. The separation by starch electrophoresis of two antibodies in sheep red cells differing in hemolytic efficiency. J Infect Dis. 1957 Mar-Apr;100(2):126–135. doi: 10.1093/infdis/100.2.126. [DOI] [PubMed] [Google Scholar]
  25. STERZL J., MANDEL L. ESTIMATION OF THE INDUCTIVE PHASE OF ANTIBODY FORMATION BY PLAQUE TECHNIQUE. Folia Microbiol (Praha) 1964 May;14:173–176. doi: 10.1007/BF02874003. [DOI] [PubMed] [Google Scholar]
  26. Schwartz S. A., Braun W. Bacteria as an Indicator of Formation of Antibodies by Single Spleen Cells in Agar. Science. 1965 Jul 9;149(3680):200–200. doi: 10.1126/science.149.3680.200. [DOI] [PubMed] [Google Scholar]

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