Abstract
Youngner, Julius S. (University of Pittsburgh, Pittsburgh, Pa.), Anne W. Scott, Jules V. Hallum, and Warren R. Stinebring. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J. Bacteriol. 92:862–868. 1966.—A comparison was made of the effects of ultraviolet (UV) irradiation or heating at 56 C on the interferon-stimulating capacity of Newcastle disease virus in primary chick embryo fibroblast (CE) cultures, in L-cell cultures, and in the intact mouse. The data obtained indicated the critical importance of the host cell system used for interferon production. Virus inactivated by UV irradiation, as well as infective virus, was an effective stimulus of interferon production in L cells and in mice, and this property persisted on continued irradiation. In contrast, in CE cell cultures, infective virus produced no interferon, whereas UV-irradiated virus produced maximal interferon titers when all infectivity was destroyed; continued irradiation resulted in a rapid loss of the interferon-stimulating capacity of the virus. Virus inactivated at 56 C did not produce interferon in CE or L-cell cultures. In the intact mouse, on the other hand, heat-inactivated virus was capable of stimulating the release of significant levels of circulating interferon.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARON S., BUCKLER C. E. CIRCULATING INTERFERON IN MICE AFTER INTRAVENOUS INJECTION OF VIRUS. Science. 1963 Sep 13;141(3585):1061–1063. doi: 10.1126/science.141.3585.1061. [DOI] [PubMed] [Google Scholar]
- BURKE D. C., ISAACS A. Some factors affecting the production of interferon. Br J Exp Pathol. 1958 Oct;39(5):452–458. [PMC free article] [PubMed] [Google Scholar]
- HENLE W., HENLE G., DEINHARDT F., BERGS V. V. Studies on persistent infections of tissue cultures. IV. Evidence for the production of an interferon in MCN cells by myxoviruses. J Exp Med. 1959 Oct 1;110:525–541. doi: 10.1084/jem.110.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HO M., BREINIG M. K. METABOLIC DETERMINANTS OF INTERFERON FORMATION. Virology. 1965 Mar;25:331–339. doi: 10.1016/0042-6822(65)90052-8. [DOI] [PubMed] [Google Scholar]
- Hallum J. V., Youngner J. S., Stinebring W. R. Interferon activity associated with high molecular weight proteins in the circulation of mice injected with endotoxin or bacteria. Virology. 1965 Nov;27(3):429–431. doi: 10.1016/0042-6822(65)90124-8. [DOI] [PubMed] [Google Scholar]
- JAGGER J. A small and inexpensive ultraviolet dose-rate meter useful in biological experiements. Radiat Res. 1961 Apr;14:394–403. [PubMed] [Google Scholar]
- MERIGAN T. C. PURIFIED INTERFERONS: PHYSICAL PROPERTIES AND SPECIES SPECIFICITY. Science. 1964 Aug 21;145(3634):811–813. doi: 10.1126/science.145.3634.811-a. [DOI] [PubMed] [Google Scholar]
- PAUCKER K., SKURSKA Z., HENLE W. Quantitative studies on viral interference in suspended L cells. I. Growth characteristics and interfering activities of vesicular stomatitis, Newcastle disease, and influenza A viruses. Virology. 1962 Jun;17:301–311. doi: 10.1016/0042-6822(62)90121-6. [DOI] [PubMed] [Google Scholar]
- STINEBRING W. R., YOUNGNER J. S. PATTERNS OF INTERFERON APPEARANCE IN MICE INFECTED WITH BACTERIA OR BACTERIAL ENDOTOXIN. Nature. 1964 Nov 14;204:712–712. doi: 10.1038/204712a0. [DOI] [PubMed] [Google Scholar]
- WAGNER R. R. Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology. 1961 Mar;13:323–337. doi: 10.1016/0042-6822(61)90152-0. [DOI] [PubMed] [Google Scholar]
- Youngner J. S., Stinebring W. R., Taube S. E. Influence of inhibitors of protein synthesis on interferon formation in mice. Virology. 1965 Dec;27(4):541–550. doi: 10.1016/0042-6822(65)90179-0. [DOI] [PubMed] [Google Scholar]