Abstract
Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925–930. 1966.—Lactobacillus casei degrades biotin when it is present in excess to products not utilizable for growth by L. plantarum or Saccharomyces cerevisiae. Degrading activity was initiated in the early stationary phase and was controlled by the pH of the medium. Nonproliferating cells, grown previously in excess biotin for 40 hr, metabolized oxybiotin and desthiobiotin as well as biotin. Cells grown in low biotin, or in excess biotin for 20 hr, did not degrade either analogue. Oxybiotin was 50% as active as biotin for growth, whereas desthiobiotin acted as a competitive inhibitor. Cells grown in excess biotin for 40 hr, but not 20 hr, overcame the inhibitory effect of desthiobiotin, when subcultured to media containing a normally inhibitory concentration of the analogue. Moreover, the level of desthiobiotin dropped rapidly during the first 4 to 6 hr before growth ensued. The data indicate that growth in excess biotin enables L. casei to degrade desthiobiotin and, thereby, to overcome the inhibitory effect of the analogue.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BIRNBAUM J., LICHSTEIN H. C. CONVERSION OF D-BIOTIN TO BIOTIN VITAMERS BY LACTOBACILLUS ARABINOSUS. J Bacteriol. 1965 Apr;89:1035–1040. doi: 10.1128/jb.89.4.1035-1040.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birnbaum J., Lichstein H. C. Metabolism of biotin and analogues of biotin by microorganisms. 3. Degradation of oxybiotin and desthiobiotin by Lactobacillus plantarum. J Bacteriol. 1966 Oct;92(4):920–924. doi: 10.1128/jb.92.4.920-924.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birnbaum J., Lichstein H. C. Metabolism of biotin and analogues of biotin by microorganisms. II. Further studies on the conversion of D-biotin to biotin vitamers by Lactobacillus plantarum. J Bacteriol. 1966 Oct;92(4):913–919. doi: 10.1128/jb.92.4.913-919.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burk D., Winzler R. J. HEAT-LABILE, AVIDIN-UNCOMBINABLE, SPECIES-SPECIFIC AND OTHER VITAMERS OF BIOTIN. Science. 1943 Jan 15;97(2507):57–60. doi: 10.1126/science.97.2507.57. [DOI] [PubMed] [Google Scholar]
- DHYSE F. G., HERTZ R. The effects of actithiazic acid on egg white-induced biotin deficiency and upon the microbial formation of biotin vitamers in the rat. Arch Biochem Biophys. 1958 Mar;74(1):7–16. doi: 10.1016/0003-9861(58)90194-2. [DOI] [PubMed] [Google Scholar]
- EISENBERG M. A. BIOTIN BIOSYNTHESIS. I. BIOTIN YIELDS AND BIOTIN VITAMERS IN CULTURES OF PHYCOMYCES BLAKESLEEANUS. J Bacteriol. 1963 Oct;86:673–680. doi: 10.1128/jb.86.4.673-680.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krueger K. K., Peterson W. H. Metabolism of Biotin and Oxybiotin by Lactobacillus pentosus 124-2. J Bacteriol. 1948 May;55(5):693–703. doi: 10.1128/jb.55.5.693-703.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lilly V. G., Leonian L. H. THE ANTI-BIOTIN EFFECT OF DESTHIOBIOTIN. Science. 1944 Mar 10;99(2567):205–206. doi: 10.1126/science.99.2567.205. [DOI] [PubMed] [Google Scholar]
- PAI C. H., LICHSTEIN H. C. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. 3. THE BIOSYNTHESIS OF (+)-BIOTIN FROM DESTHIOBIOTIN AND ITS CONTROL IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 12;100:43–49. doi: 10.1016/0304-4165(65)90425-3. [DOI] [PubMed] [Google Scholar]
- Pilgrim F. J., Axelrod A. E., Winnick T., Hofmann K. THE MICROBIOLOGICAL ACTIVITY OF AN OXYGEN ANALOG OF BIOTIN. Science. 1945 Jul 13;102(2637):35–36. doi: 10.1126/science.102.2637.35. [DOI] [PubMed] [Google Scholar]
- Rose A. H., Ilahi M., Kelemen M. V. Studies on the biosynthesis of biotin. Production of biotin and biotin-like compounds by a pseudomonad. Biochem J. 1965 Aug;96(2):319–327. doi: 10.1042/bj0960319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waller J. R., Lichstein H. C. Biotin transport and accumulation by cells of Lactobacillus plantarum. I. General properties of the system. J Bacteriol. 1965 Oct;90(4):843–852. doi: 10.1128/jb.90.4.843-852.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]