Abstract
Matney, Thomas S. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas), and Joan C. Suit. Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escherichia coli. J. Bacteriol. 92:960–966. 1966.—A procedure was developed for phasing large-volume cultures of Escherichia coli K-12 with regard to cell division. The method consists of permitting the bacteria to exhaust a growth-limiting supply of a required amino acid, starving the culture, resupplementing with an excess of the amino acid, and following the ensuing growth by usual counting procedures.
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Selected References
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haas F. L., Doudney C. O. A RELATION OF NUCLEIC ACID SYNTHESIS TO RADIATION-INDUCED MUTATION FREQUENCY IN BACTERIA. Proc Natl Acad Sci U S A. 1957 Oct 15;43(10):871–883. doi: 10.1073/pnas.43.10.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harrison A. P., Jr Thymine incorporation and metabolism by various classes of thymine-less bacteria. J Gen Microbiol. 1965 Dec;41(3):321–333. doi: 10.1099/00221287-41-3-321. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
- Matney T. S., Goldschmidt E. P., Erwin N. S., Scroggs R. A. A preliminary map of genomic sites for F-attachment in Escherichia coli K12. Biochem Biophys Res Commun. 1964 Oct 14;17(3):278–281. doi: 10.1016/0006-291x(64)90397-3. [DOI] [PubMed] [Google Scholar]
- OGUR M., ROSEN G. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch Biochem. 1950 Feb;25(2):262–276. [PubMed] [Google Scholar]
- STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]