Table 1.

Proteins of T. cruzi epimastigote reservosomes identified by proteomic analysis and with annotated function or cellular localization

Hit number	Protein group	GenBank accession number	Function/Cellular localization	Transmembrane domain (number of domains)1
	(1) Protein metabolism
1	Serine carboxypeptidase	XP_803003	Lysosomal, peptidase family S10	N
2	Lysosomal alpha-mannosidase	XP_819105	Lysosomal, carbohydrate metabolism	N
3	Histidine ammonia-lyase	XP_820336	Convert histidine into ammonia and urocanic acid	N
4	Cysteine proteinase	XP_811957	Lysosomal, similar to endopeptidase papain	Y (1)
5	Cysteine peptidase	XP_821954	Lysosomal integral to membrane, proteolysis	Y (1)
6	S-Adenosylhomocysteine hydrolase	XP_809153	Adenosylhomocysteine hydrolase activity	N
7	Cysteine peptidase C	XP_812694	Lysosomal, proteolysis	N
8	Calpain cysteine peptidase	XP_816696	Calcium-dependent cysteine-type endopeptidase activity	N
9	Membrane-bound acid phosphatase	XP_808329	Catalytic activity	N
10	Membrane-bound acid phosphatase 2	XP_815570	Lysosomal, acid phosphatase activity	Y (1)
11	Serine carboxypeptidase S28	XP_818592	Peptidase S28	N
12	Cruzipain	AAF75547	Cysteine peptidase, clan CA, family C1	N
13	Tartrate-resistant acid phosphatase type 5	XP_811082	Metallophosphoesterase	Y (2)
14	Ubiquitin hydrolase	XP_821344	Protein deubiquitination	N
15	Glutamamyl carboxypeptidase	XP_804169	Metallopeptidase activity, proteolysis	N
16	Peptidase M20/M25/M40	XP_813635	Metallopeptidase activity	N
17	Chagasin	2FO8	Cysteine Protease Inhibitor	N
	(2) Endosomal/lysosomal membrane proteins
18	Golgi/lysosome glycoprotein	XP_817916	Golgi/lysosome membrane protein	Y (1)
19	Lysosomal/endosomal membrane protein p67	XP_803464	Lysosomal/endosomal membrane protein	Y (1)
20	Endosomal integral membrane protein	XP_817908	Integral to endosomal membrane	Y (9)
21	Endosomal integral membrane protein	XP_813892	Endosomal protein	Y (11)
22	Huntingtin interacting protein (HIP)	XP_816878	Endocytosis, metal ion binding	Y (4)
	(3) Vesicular traffic
23	Vesicle-associated membrane protein	XP_816323	Integral to plasma membrane, vesicle targeting	Y (1)
24	Vesicle-associated membrane protein	XP_812489	Exocytosis	Y (1)
25	Rab1	XP_805990	ER, small GTPase mediated signal transduction	N
26	Rab-2a	XP_809735	ER, Small GTPase mediated signal transduction	N
27	COP-coated vesicle membrane protein erv25	XP_821489	Coated vesicle membrane protein	Y (1)
28	COP-coated vesicle membrane protein gp25l	XP_805665	ER to Golgi vesicle-mediated transport	Y (1)
29	Small GTP-binding protein	XP_808772	ER to Golgi vesicle-mediated transport	N
30	Rab7	XP_821619	Small GTPase mediated signal transduction	N
31	Rab18	XP_818013	Small GTPase mediated signal transduction	N
32	Mu-adaptin 1	XP_818899	Adaptor complexes 1 subunit	N
	(4) pumps and channel proteins
33	P-type H+-atpase (tcha3)	XP_812631	ATPase, transmembrane proton transport	Y (6)
34	P-type H+-atpase (tcha1)	XP_804256	ATPase, transmembrane proton transport	Y (3)
35	P-type H+-atpase (tcha2)	AAL87542.1	ATPase, transmembrane proton transport	Y (8)
36	ABC transporter	XP_818638	ATPase, transmembrane transport of substances	Y (12)
37	P-glycoprotein	AAC09044.1	ABC transporter protein	Y (8)
38	Multidrug resistance protein E	XP_815145	Xenobiotic-transporting ATPase activity	Y (4)
39	Proton motive atpase	AAB70152	Cation transport ATPase, P-type ATPase	N
40	Vacuolar-type proton pyrophosphatase	XP_813460	Hydrogen-translocating pyrophosphatase activity	Y (15)
41	Ferric reductase	XP_808251	Electron transport, oxidoreductase activity	Y (12)
42	Chloride channel protein	XP_816207	Voltage-gated chloride channel activity	Y (10)
43	Hexose transporter	XP_814821	Glucose transmembrane transporter activity	Y (12)
44	V-type atpase, A subunit	XP_807670	Lysosomal proton-transporting V-type ATPase complex	N
45	Folate/pteridine transporter	XP_810759	Biopterin transport	Y (12)
46	Vacuolar ATP synthase	XP_808900	Hydrogen-exporting ATP activity, phosphorylative mechanism	N
47	Amino acid permease	XP_802539	Transmembrane amino acid transporter protein	Y (10)
48	MFS transporter	XP_804323	Transport small solutes across membranes	Y (7)
49	Amino acid permease/transporter	XP_819801	Amino acid transport	Y (10)
50	Calcium-translocating P-type atpase	XP_814228	Calcium-transporting ATPase activity	Y (3)
	(5) Cell surface proteins
51	Surface protease GP63	XP_821289	Neutral zinc metallopeptidases	Y (1)
52	Dispersed gene family protein 1 (DGF-1)	XP_820404	Cell surface protein	Y (9)
53	Procyclic form surface glycoprotein	XP_803886		Y (3)
54	Kinetoplastid membrane protein KMP-11	XP_808865	kinetoplastid membrane protein	N
	(6) Lipid metabolism
55	Sterol 24-c-methyltransferase	XP_802864	Biosynthesis of ergosterol	N
56	Fatty acid elongase	XP_813971	Long-chain fatty acid biosynthetic process	Y (7)
57	3-oxo-5-alpha-steroid 4-dehydrogenase	XP_802815	Very-long-chain fatty acid metabolic process	Y (4)
58	Fatty acyl coa synthetase	XP_817096	Long-chain-fatty-acid-CoA ligase activity	N
59	Serine-palmitoyl-coa transferase	XP_812739	Sphingolipid metabolic process	N
60	Sterol C-24 reductase	XP_816888	Ergosterol biosynthesis	Y (9)
61	Phospholipid-translocating atpase	XP_817305	Phospholipid-translocating P-type ATPase, flippase	Y (12)
62	Fatty acyl coa synthetase 2	XP_802708	Long-chain fatty acid activation	N
63	C-8 sterol isomerase	XP_821186	Ergosterol biosynthetic process	Y (1)
64	Fatty acyl coa syntetase 1	XP_812351	Fatty acid metabolic process	N
65	Phosphatidylcholine:ceramide cholinephosphotransferase	XP_821506	Sphingomyelin biosynthetic process	Y (5)
66	Phosphatidic acid phosphatase protein	XP_814714	Phospholipid metabolic process	Y (5)
67	Lipase domain protein	XP_814907	Lipid metabolic process	Y (7)
	(7) Carbohydrate metabolism
68	UDP-Gal or UDP-glcnac-dependent glycosyltransferase	XP_814599	Transfer galactose to GlcNAc terminal chains	N
69	Enolase	XP_819700	Glycolysis, phosphopyruvate hydratase activity	N
70	Hexokinase	XP_808992	Carbohydrate metabolic process	N
71	Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB	XP_811964	Protein amino acid N-linked glycosylation	N
72	Beta galactofuranosyl glycosyltransferase	XP_810392		N
73	Lectin	XP_807732	Mannose binding	N
74	Glucokinase 1	XP_821474	Glucokinase activity	N
75	Glucosamine-6-phosphate isomerase	XP_807857	N-acetylglucosamine catabolic process	N
	(8) Cell signaling
76	Protein kinase	XP_807538	Protein amino acid phosphorilation	N
77	Protein tyrosine phosphatase	XP_816679	Protein tyrosine phosphatase activator factor	N
78	Serine/threonine protein kinase	XP_822060	Protein amino acid phosphorilation	N
79	Serine/threonine protein phosphatase	XP_809469	Calcium-dependent protein serine/threonine phosphatase	Y (2)
80	Rac serine-threonine kinase	XP_815140		N
81	Regulatory subunit of protein kinase a	XP_810045	Protein kinase activity	N
82	Casein kinase delta isoform	XP_803179	Casein kinase activity	N
83	Activated protein kinase C receptor	XP_817733	Protein kinase C binding	N
84	Casein kinase	XP_803179	Protein amino acid phosphorylation	N
	(9) Cytoskeleton-associated proteins
85	Beta tubulin	XP_816690	Microtubule cytoskeleton organization and biogenesis	N
86	Alpha tubulin	XP_802499	Microtubule cytoskeleton organization and biogenesis	N
87	Actin	XP_807262	Microfilament organization and biogenesis	N
88	MCAK-like kinesin	XP_813221	Microtubule associated complex	N
89	Cytoskeleton-associated protein CAP5.5	XP_809814	Cytoskeleton-associated protein	N
90	Cofilin/actin depolymerizing factor	XP_806100	Actin filament organization
91	I/6 autoantigen	XP_813169	Calcium ion binding, structural constituent of cytoskeleton	N
	(10) Cytosolic proteins
92	Elongation factor alpha G5	AAU47272	Calcium mediated signaling, microtubule-based process	N
93	Tryparedoxin peroxidase	XP_802393	Trypanothione-disulfide reductase activity	N
94	Hypoxanthine-guanine phosphoribosyltransferase	XP_813397	Nucleotide transport and metabolism	N
95	Methylthioadenosine phosphorylase	XP_819927	Amino acid salvage	N
96	6-phosphogluconate dehydrogenase	XP_808031	Pentose-phosphate shunt	N
97	Disulfide isomerase	XP_821173	Protein folding	N
	(11) Glycosomal proteins
98	Glutamate dehydrogenase	XP_818855	Glutamate biosynthesis, using glutamate synthase (NADPH)	N
99	Glycosomal phosphoenolpyruvate carboxykinase	XP_810815	Gluconeosenesis, microtubule binding	N
100	Glyceraldehyde 3-phosphate dehydrogenase	XP_812137	Glycolysis	N
101	NADH-dependent fumarate reductase	XP_810232	Glucose metabolic process	N
102	Gim5A protein	XP_821649	Glycosomal membrane	N
103	Glycosomal malate dehydrogenase	XP_812467		N
104	Fructose-bisphosphate aldolase	XP_809369	Fructose-bisphosphate aldolase activity	N
105	Glucose-6-phosphate isomerase	XP_821969	Gluconeogenesis, glycolysis	N
106	6-phospho-1-fructokinase	XP_822053	Glycolysis	N
	(12) Mitochondrial proteins
107	Heat shock 70 kda protein	XP_806221	Membrane protein folding	N
108	Malate dehydrogenase	XP_809210	Tricarboxilyc acid cycle	N
109	Mitochondrial processing peptidase, beta subunit	XP_816361	Mitochondrial processing peptidase activity	N
110	Pyruvate dehydrogenase E1 beta subunit	XP_811646	Pyruvate dehydrogenase (acetyl-transferring) activity	N
111	Succinyl-coa ligase [GDP-forming] beta-chain	XP_802641	Succinate-CoA ligase (GDP-forming) activity	N
112	P22 protein precursor	XP_813311	Transcription regulator activity	N
113	ADP, ATP carrier protein 1	XP_812264	Transport, mitochondrial inner membrane	Y (3)
114	RNA-binding protein 2	XP_818766	Mitochondrial RNA binding	N
115	Acyl carrier protein	XP_814961	Fatty acid biosynthetic process	N
116	Oligo_U binding protein TBRGG1	XP_809536		N
117	ATP synthase, epsilon chain	XP_818987	Proton-transporting ATP synthase complex	N
118	Chaperonin HSP60/CNP60	XP_804030	Protein import into mitochondrial matrix	N
119	Succinyl-coa ligase [GDP-forming] beta-chain	XP_818583	Tricarboxylic acid cycle	N
120	Aldehyde dehydrogenase	XP_813115	Oxidation of aldehydes	N
121	ADP, ATP carrier protein 1	XP_819458	Mitochodria inner membrane, transport	Y (3)
	(13) others
122	Glucose-regulated protein 78	XP_809817	Endoplasmic reticulum, protein folding	N
123	Calreticulin	XP_812571	Protein folding, sugar binding	N
124	Calcium-binding protein	XP_808304	Flagellar calcium binding protein	N
125	Malic enzyme	XP_814410	Malate metabolic process	N
126	Cyclophilin A	XP_821578	Protein folding	N
127	S-phase kinase-associated protein	XP_806972	Ubiquitin-dependent protein catabolic process	N
128	Calmodulin	XP_874377	Calcium ion binding	N
129	Centrin	XP_813165	Basal body, calcium ion binding	N

¹N, no; Y, yes