Table 1.
Hit number |
Protein group | GenBank accession number |
Function/Cellular localization | Transmembrane domain (number of domains)1 |
---|---|---|---|---|
(1) Protein metabolism | ||||
1 | Serine carboxypeptidase | XP_803003 | Lysosomal, peptidase family S10 | N |
2 | Lysosomal alpha-mannosidase | XP_819105 | Lysosomal, carbohydrate metabolism | N |
3 | Histidine ammonia-lyase | XP_820336 | Convert histidine into ammonia and urocanic acid | N |
4 | Cysteine proteinase | XP_811957 | Lysosomal, similar to endopeptidase papain | Y (1) |
5 | Cysteine peptidase | XP_821954 | Lysosomal integral to membrane, proteolysis | Y (1) |
6 | S-Adenosylhomocysteine hydrolase | XP_809153 | Adenosylhomocysteine hydrolase activity | N |
7 | Cysteine peptidase C | XP_812694 | Lysosomal, proteolysis | N |
8 | Calpain cysteine peptidase | XP_816696 | Calcium-dependent cysteine-type endopeptidase activity | N |
9 | Membrane-bound acid phosphatase | XP_808329 | Catalytic activity | N |
10 | Membrane-bound acid phosphatase 2 | XP_815570 | Lysosomal, acid phosphatase activity | Y (1) |
11 | Serine carboxypeptidase S28 | XP_818592 | Peptidase S28 | N |
12 | Cruzipain | AAF75547 | Cysteine peptidase, clan CA, family C1 | N |
13 | Tartrate-resistant acid phosphatase type 5 | XP_811082 | Metallophosphoesterase | Y (2) |
14 | Ubiquitin hydrolase | XP_821344 | Protein deubiquitination | N |
15 | Glutamamyl carboxypeptidase | XP_804169 | Metallopeptidase activity, proteolysis | N |
16 | Peptidase M20/M25/M40 | XP_813635 | Metallopeptidase activity | N |
17 | Chagasin | 2FO8 | Cysteine Protease Inhibitor | N |
(2) Endosomal/lysosomal membrane proteins | ||||
18 | Golgi/lysosome glycoprotein | XP_817916 | Golgi/lysosome membrane protein | Y (1) |
19 | Lysosomal/endosomal membrane protein p67 | XP_803464 | Lysosomal/endosomal membrane protein | Y (1) |
20 | Endosomal integral membrane protein | XP_817908 | Integral to endosomal membrane | Y (9) |
21 | Endosomal integral membrane protein | XP_813892 | Endosomal protein | Y (11) |
22 | Huntingtin interacting protein (HIP) | XP_816878 | Endocytosis, metal ion binding | Y (4) |
(3) Vesicular traffic | ||||
23 | Vesicle-associated membrane protein | XP_816323 | Integral to plasma membrane, vesicle targeting | Y (1) |
24 | Vesicle-associated membrane protein | XP_812489 | Exocytosis | Y (1) |
25 | Rab1 | XP_805990 | ER, small GTPase mediated signal transduction | N |
26 | Rab-2a | XP_809735 | ER, Small GTPase mediated signal transduction | N |
27 | COP-coated vesicle membrane protein erv25 | XP_821489 | Coated vesicle membrane protein | Y (1) |
28 | COP-coated vesicle membrane protein gp25l | XP_805665 | ER to Golgi vesicle-mediated transport | Y (1) |
29 | Small GTP-binding protein | XP_808772 | ER to Golgi vesicle-mediated transport | N |
30 | Rab7 | XP_821619 | Small GTPase mediated signal transduction | N |
31 | Rab18 | XP_818013 | Small GTPase mediated signal transduction | N |
32 | Mu-adaptin 1 | XP_818899 | Adaptor complexes 1 subunit | N |
(4) pumps and channel proteins | ||||
33 | P-type H+-atpase (tcha3) | XP_812631 | ATPase, transmembrane proton transport | Y (6) |
34 | P-type H+-atpase (tcha1) | XP_804256 | ATPase, transmembrane proton transport | Y (3) |
35 | P-type H+-atpase (tcha2) | AAL87542.1 | ATPase, transmembrane proton transport | Y (8) |
36 | ABC transporter | XP_818638 | ATPase, transmembrane transport of substances | Y (12) |
37 | P-glycoprotein | AAC09044.1 | ABC transporter protein | Y (8) |
38 | Multidrug resistance protein E | XP_815145 | Xenobiotic-transporting ATPase activity | Y (4) |
39 | Proton motive atpase | AAB70152 | Cation transport ATPase, P-type ATPase | N |
40 | Vacuolar-type proton pyrophosphatase | XP_813460 | Hydrogen-translocating pyrophosphatase activity | Y (15) |
41 | Ferric reductase | XP_808251 | Electron transport, oxidoreductase activity | Y (12) |
42 | Chloride channel protein | XP_816207 | Voltage-gated chloride channel activity | Y (10) |
43 | Hexose transporter | XP_814821 | Glucose transmembrane transporter activity | Y (12) |
44 | V-type atpase, A subunit | XP_807670 | Lysosomal proton-transporting V-type ATPase complex | N |
45 | Folate/pteridine transporter | XP_810759 | Biopterin transport | Y (12) |
46 | Vacuolar ATP synthase | XP_808900 | Hydrogen-exporting ATP activity, phosphorylative mechanism | N |
47 | Amino acid permease | XP_802539 | Transmembrane amino acid transporter protein | Y (10) |
48 | MFS transporter | XP_804323 | Transport small solutes across membranes | Y (7) |
49 | Amino acid permease/transporter | XP_819801 | Amino acid transport | Y (10) |
50 | Calcium-translocating P-type atpase | XP_814228 | Calcium-transporting ATPase activity | Y (3) |
(5) Cell surface proteins | ||||
51 | Surface protease GP63 | XP_821289 | Neutral zinc metallopeptidases | Y (1) |
52 | Dispersed gene family protein 1 (DGF-1) | XP_820404 | Cell surface protein | Y (9) |
53 | Procyclic form surface glycoprotein | XP_803886 | Y (3) | |
54 | Kinetoplastid membrane protein KMP-11 | XP_808865 | kinetoplastid membrane protein | N |
(6) Lipid metabolism | ||||
55 | Sterol 24-c-methyltransferase | XP_802864 | Biosynthesis of ergosterol | N |
56 | Fatty acid elongase | XP_813971 | Long-chain fatty acid biosynthetic process | Y (7) |
57 | 3-oxo-5-alpha-steroid 4-dehydrogenase | XP_802815 | Very-long-chain fatty acid metabolic process | Y (4) |
58 | Fatty acyl coa synthetase | XP_817096 | Long-chain-fatty-acid-CoA ligase activity | N |
59 | Serine-palmitoyl-coa transferase | XP_812739 | Sphingolipid metabolic process | N |
60 | Sterol C-24 reductase | XP_816888 | Ergosterol biosynthesis | Y (9) |
61 | Phospholipid-translocating atpase | XP_817305 | Phospholipid-translocating P-type ATPase, flippase | Y (12) |
62 | Fatty acyl coa synthetase 2 | XP_802708 | Long-chain fatty acid activation | N |
63 | C-8 sterol isomerase | XP_821186 | Ergosterol biosynthetic process | Y (1) |
64 | Fatty acyl coa syntetase 1 | XP_812351 | Fatty acid metabolic process | N |
65 | Phosphatidylcholine:ceramide cholinephosphotransferase | XP_821506 | Sphingomyelin biosynthetic process | Y (5) |
66 | Phosphatidic acid phosphatase protein | XP_814714 | Phospholipid metabolic process | Y (5) |
67 | Lipase domain protein | XP_814907 | Lipid metabolic process | Y (7) |
(7) Carbohydrate metabolism | ||||
68 | UDP-Gal or UDP-glcnac-dependent glycosyltransferase | XP_814599 | Transfer galactose to GlcNAc terminal chains | N |
69 | Enolase | XP_819700 | Glycolysis, phosphopyruvate hydratase activity | N |
70 | Hexokinase | XP_808992 | Carbohydrate metabolic process | N |
71 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB | XP_811964 | Protein amino acid N-linked glycosylation | N |
72 | Beta galactofuranosyl glycosyltransferase | XP_810392 | N | |
73 | Lectin | XP_807732 | Mannose binding | N |
74 | Glucokinase 1 | XP_821474 | Glucokinase activity | N |
75 | Glucosamine-6-phosphate isomerase | XP_807857 | N-acetylglucosamine catabolic process | N |
(8) Cell signaling | ||||
76 | Protein kinase | XP_807538 | Protein amino acid phosphorilation | N |
77 | Protein tyrosine phosphatase | XP_816679 | Protein tyrosine phosphatase activator factor | N |
78 | Serine/threonine protein kinase | XP_822060 | Protein amino acid phosphorilation | N |
79 | Serine/threonine protein phosphatase | XP_809469 | Calcium-dependent protein serine/threonine phosphatase | Y (2) |
80 | Rac serine-threonine kinase | XP_815140 | N | |
81 | Regulatory subunit of protein kinase a | XP_810045 | Protein kinase activity | N |
82 | Casein kinase delta isoform | XP_803179 | Casein kinase activity | N |
83 | Activated protein kinase C receptor | XP_817733 | Protein kinase C binding | N |
84 | Casein kinase | XP_803179 | Protein amino acid phosphorylation | N |
(9) Cytoskeleton-associated proteins | ||||
85 | Beta tubulin | XP_816690 | Microtubule cytoskeleton organization and biogenesis | N |
86 | Alpha tubulin | XP_802499 | Microtubule cytoskeleton organization and biogenesis | N |
87 | Actin | XP_807262 | Microfilament organization and biogenesis | N |
88 | MCAK-like kinesin | XP_813221 | Microtubule associated complex | N |
89 | Cytoskeleton-associated protein CAP5.5 | XP_809814 | Cytoskeleton-associated protein | N |
90 | Cofilin/actin depolymerizing factor | XP_806100 | Actin filament organization | |
91 | I/6 autoantigen | XP_813169 | Calcium ion binding, structural constituent of cytoskeleton | N |
(10) Cytosolic proteins | ||||
92 | Elongation factor alpha G5 | AAU47272 | Calcium mediated signaling, microtubule-based process | N |
93 | Tryparedoxin peroxidase | XP_802393 | Trypanothione-disulfide reductase activity | N |
94 | Hypoxanthine-guanine phosphoribosyltransferase | XP_813397 | Nucleotide transport and metabolism | N |
95 | Methylthioadenosine phosphorylase | XP_819927 | Amino acid salvage | N |
96 | 6-phosphogluconate dehydrogenase | XP_808031 | Pentose-phosphate shunt | N |
97 | Disulfide isomerase | XP_821173 | Protein folding | N |
(11) Glycosomal proteins | ||||
98 | Glutamate dehydrogenase | XP_818855 | Glutamate biosynthesis, using glutamate synthase (NADPH) | N |
99 | Glycosomal phosphoenolpyruvate carboxykinase | XP_810815 | Gluconeosenesis, microtubule binding | N |
100 | Glyceraldehyde 3-phosphate dehydrogenase | XP_812137 | Glycolysis | N |
101 | NADH-dependent fumarate reductase | XP_810232 | Glucose metabolic process | N |
102 | Gim5A protein | XP_821649 | Glycosomal membrane | N |
103 | Glycosomal malate dehydrogenase | XP_812467 | N | |
104 | Fructose-bisphosphate aldolase | XP_809369 | Fructose-bisphosphate aldolase activity | N |
105 | Glucose-6-phosphate isomerase | XP_821969 | Gluconeogenesis, glycolysis | N |
106 | 6-phospho-1-fructokinase | XP_822053 | Glycolysis | N |
(12) Mitochondrial proteins | ||||
107 | Heat shock 70 kda protein | XP_806221 | Membrane protein folding | N |
108 | Malate dehydrogenase | XP_809210 | Tricarboxilyc acid cycle | N |
109 | Mitochondrial processing peptidase, beta subunit | XP_816361 | Mitochondrial processing peptidase activity | N |
110 | Pyruvate dehydrogenase E1 beta subunit | XP_811646 | Pyruvate dehydrogenase (acetyl-transferring) activity | N |
111 | Succinyl-coa ligase [GDP-forming] beta-chain | XP_802641 | Succinate-CoA ligase (GDP-forming) activity | N |
112 | P22 protein precursor | XP_813311 | Transcription regulator activity | N |
113 | ADP, ATP carrier protein 1 | XP_812264 | Transport, mitochondrial inner membrane | Y (3) |
114 | RNA-binding protein 2 | XP_818766 | Mitochondrial RNA binding | N |
115 | Acyl carrier protein | XP_814961 | Fatty acid biosynthetic process | N |
116 | Oligo_U binding protein TBRGG1 | XP_809536 | N | |
117 | ATP synthase, epsilon chain | XP_818987 | Proton-transporting ATP synthase complex | N |
118 | Chaperonin HSP60/CNP60 | XP_804030 | Protein import into mitochondrial matrix | N |
119 | Succinyl-coa ligase [GDP-forming] beta-chain | XP_818583 | Tricarboxylic acid cycle | N |
120 | Aldehyde dehydrogenase | XP_813115 | Oxidation of aldehydes | N |
121 | ADP, ATP carrier protein 1 | XP_819458 | Mitochodria inner membrane, transport | Y (3) |
(13) others | ||||
122 | Glucose-regulated protein 78 | XP_809817 | Endoplasmic reticulum, protein folding | N |
123 | Calreticulin | XP_812571 | Protein folding, sugar binding | N |
124 | Calcium-binding protein | XP_808304 | Flagellar calcium binding protein | N |
125 | Malic enzyme | XP_814410 | Malate metabolic process | N |
126 | Cyclophilin A | XP_821578 | Protein folding | N |
127 | S-phase kinase-associated protein | XP_806972 | Ubiquitin-dependent protein catabolic process | N |
128 | Calmodulin | XP_874377 | Calcium ion binding | N |
129 | Centrin | XP_813165 | Basal body, calcium ion binding | N |
N, no; Y, yes