Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Oct;92(4):983–989. doi: 10.1128/jb.92.4.983-989.1966

Persistent, Noncytocidal Viral Infection: Nonsynchrony of Viral and Cellular Multiplication

Duard L Walker a, Ping-Ping Chang a, Robert L Northrop a,1, Harry C Hinze a
PMCID: PMC276365  PMID: 5926763

Abstract

Walker, Duard L. (University of Wisconsin Medical School, Madison), Ping-Ping Chang, Robert L. Northrop, and Harry C. Hinze. Persistent, noncytocidal viral infection: nonsynchrony of viral and cellular multiplication. J. Bacteriol. 92:983–989. 1966.—In cultures of human conjunctive cells persistently infected with mumps virus (C-M cultures), the degree to which viral multiplication is linked to cell multiplication was examined. First, cell multiplication was inhibited. This resulted in a 10-fold increase in virus-excreting cells in the culture and an 80-fold increase in virus in the medium as compared with vigorously growing control cultures. Second, by use of elevated incubation temperature, virus multiplication was inhibited without slowing multiplication of the cells, and uninfected cells that appeared in the culture were protected by virus antiserum. This resulted in accumulation of antigen-free cells and, in one experiment, in elimination of the virus. Evidence indicated that uninfected cells accumulated as a result of dilution of virus by repeated cell divisions, but not as a result of selection of uninfected cells. These data indicate that viral multiplication in the C-M system is not closely linked to cell multiplication and that each can proceed at a rate different from the other.

Full text

PDF
983

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDZHAPARIDZE O. G., BOGOMOLOVA N. N. Interaction of tick-borne encephalitis virus and susceptible cells in vitro. II. Latent infection of cells. Probl Virol. 1961;6:369–373. [PubMed] [Google Scholar]
  2. CHOPPIN P. W. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE. Virology. 1964 Jun;23:224–233. doi: 10.1016/0042-6822(64)90286-7. [DOI] [PubMed] [Google Scholar]
  3. COLE F. E., Jr, HETRICK F. M. PERSISTENT INFECTION OF HUMAN CONJUNCTIVA CELL CULTURES BY MYXOVIRUS PARAINFLUENZA 3. Can J Microbiol. 1965 Jun;11:513–521. doi: 10.1139/m65-068. [DOI] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. FERNANDES M. V., WIKTOR T. J., KOPROWSKI H. ENDOSYMBIOTIC RELATIONSHIP BETWEEN ANIMAL VIRUSES AND HOST CELLS. A STUDY OF RABIES VIRUS IN TISSUE CULTURE. J Exp Med. 1964 Dec 1;120:1099–1116. doi: 10.1084/jem.120.6.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HANAFUSA H., HANAFUSA T., RUBIN H. The defectiveness of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1963 Apr;49:572–580. doi: 10.1073/pnas.49.4.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ISHIDA N., HOMMA M., OSATO T., HINUMA Y., MIYAMOTO T. PERSISTENT INFECTION IN HELA CELLS WITH HEMADSORPTION VIRUS TYPE 2. Virology. 1964 Dec;24:670–672. doi: 10.1016/0042-6822(64)90224-7. [DOI] [PubMed] [Google Scholar]
  8. MAGUIRE T., MILES J. A. THE ARBOVIRUS CARRIER STATE IN TISSUE CULTURES. Arch Gesamte Virusforsch. 1965;15:457–474. doi: 10.1007/BF01245238. [DOI] [PubMed] [Google Scholar]
  9. Maassab H. F., Veronelli J. A. Characteristics of serially propagated monkey kidney cell cultures with persistent rubella infection. J Bacteriol. 1966 Jan;91(1):436–441. doi: 10.1128/jb.91.1.436-441.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Macpherson I. Reversion in Hamster Cells Transformed by Rous Sarcoma Virus. Science. 1965 Jun 25;148(3678):1731–1733. doi: 10.1126/science.148.3678.1731. [DOI] [PubMed] [Google Scholar]
  11. Marcus P. I., Carver D. H. Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interference. Science. 1965 Aug 27;149(3687):983–986. doi: 10.1126/science.149.3687.983. [DOI] [PubMed] [Google Scholar]
  12. Miyamoto T., Hinuma Y., Ishida N. Intracellular transfer of hemadsorption type 2 virus antigen during persistent infection of HeLa cell cultures. Virology. 1965 Sep;27(1):28–36. doi: 10.1016/0042-6822(65)90140-6. [DOI] [PubMed] [Google Scholar]
  13. RUSTIGIAN R. A carrier state in HeLa cells with measles virus (Edmonston strain) apparently associated with non-infectious virus. Virology. 1962 Jan;16:101–104. doi: 10.1016/0042-6822(62)90212-x. [DOI] [PubMed] [Google Scholar]
  14. TEMIN H. M. Separation of morphological conversion and virus production in Rous sarcoma virus infection. Cold Spring Harb Symp Quant Biol. 1962;27:407–414. doi: 10.1101/sqb.1962.027.001.038. [DOI] [PubMed] [Google Scholar]
  15. TEMIN H. M. THE PARTICIPATION OF DNA IN ROUS SARCOMA VIRUS PRODUCTION. Virology. 1964 Aug;23:486–494. doi: 10.1016/0042-6822(64)90232-6. [DOI] [PubMed] [Google Scholar]
  16. WALKER D. L., HINZE H. C. A carrier state of mumps virus in human conjunctiva cells. I. General characteristics. J Exp Med. 1962 Nov 1;116:739–750. doi: 10.1084/jem.116.5.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WALKER D. L., HINZE H. C. A carrier state of mumps virus in human conjunctiva cells. II. Observations on intercellular transfer of virus and virus release. J Exp Med. 1962 Nov 1;116:751–758. doi: 10.1084/jem.116.5.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
  19. Wiktor T. J., Fernandes M. V., Koprowski H. Detection of a lymphocytic choriomeningitis component in rabies virus preparations. J Bacteriol. 1965 Nov;90(5):1494–1495. doi: 10.1128/jb.90.5.1494-1495.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES