Abstract
Stutzenberger, Fred J. (Michigan State University, East Lansing), Charles L. San Clemente, and Dharam V. Vadehra. Nephelometric assay of staphylococcal coagulase. J. Bacteriol. 92:1005–1009. 1966.—Clotting of fibrinogen by staphylococcal coagulase was accompanied by an increase in light scattering; this property was used as a basis for a new nephelometric method. Reaction rates, which were now easily and precisely measured, were found to be directly proportional to coagulase concentration, when optimal conditions were maintained. These conditions included pH and concentrations of fibrinogen, coagulase-reacting factor, and sodium chloride in the reaction mixture. A standardized procedure for the assay is outlined, and a unit for the expression of activity is proposed.
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Selected References
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- BLAIR J. E., CARR M. The techniques and interpretation of phage typing of staphylococci. J Lab Clin Med. 1960 Apr;55:650–662. [PubMed] [Google Scholar]
- DUTHIE E. S., HAUGHTON G. Purification of free staphylococcal coagulase. Biochem J. 1958 Sep;70(1):125–134. doi: 10.1042/bj0700125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAIDANOVA N. V. ISSLEDOVANIE KATALITICHESKIKH SVO ISTV TROMBINA I KOAGULAZY STAPHULOCOCCUS AUREUS. Biokhimiia. 1964 May-Jun;29:432–438. [PubMed] [Google Scholar]
- SHEPPARD E., IMPERANTE J., WRIGHT I. S. The effect of phosphorylated hesperidin on the polymerization of fibrinogen as studied by light scattering. J Biol Chem. 1955 Feb;212(2):837–846. [PubMed] [Google Scholar]
- STEINER R. F., LAKI K. Light scattering studies on the clotting of fibrinogen. Arch Biochem Biophys. 1951 Nov;34(1):24–37. doi: 10.1016/s0003-9861(51)80005-5. [DOI] [PubMed] [Google Scholar]