Abstract
Organick, Avrum B. (Marquette University School of Medicine, Milwaukee, Wis.), Kenneth A. Siegesmund, and Irving I. Lutsky. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J. Bacteriol. 92:1164–1176. 1966.—Lesions in lungs of gnotobiotic mice inoculated intranasally with Mycoplasma pulmonis were examined by electron microscopy after osmic acid fixation. At 1 week after infection, mycoplasma cells were found in large numbers in the bronchi at the surface of bronchial epithelial cells and, in smaller numbers, in the alveoli where active phagocytosis by polymorphonuclear leukocytes (PMN) occurred. Cytopathic changes in underlying bronchial epithelial cells, not apparent by light microscopy, were observed. At 3 weeks after infection, mycoplasma cells were rarely seen in the bronchi, and were no longer seen free in the alveolar spaces or within PMN. Lungs examined after glutaraldehyde fixation 1 week after infection confirmed the presence of mycoplasma cells in the alveolar spaces and within phagocytic vacuoles of PMN, but also revealed numerous ring forms within granular pneumocytes. These forms seemed to represent intracytoplasmic developmental stages of M. pulmonis, in which elementary bodies appeared in large numbers.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson D. R., Barile M. F. Ultrastructure of Mycoplasma hominis. J Bacteriol. 1965 Jul;90(1):180–192. doi: 10.1128/jb.90.1.180-192.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAJANI A. S., CLYDE W. A., Jr, DENNY F. W. EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT). J Exp Med. 1965 Jun 1;121:1071–1086. doi: 10.1084/jem.121.6.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOMERMUTH C. H., NIELSEN M. H., FREUNDT E. A., BIRCH-ANDERSEN A. ULTRASTRUCTURE OF MYCOPLASMA SPECIES. J Bacteriol. 1964 Sep;88:727–744. doi: 10.1128/jb.88.3.727-744.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDWARDS G. A., FOGH J. Fine structure of pleuropneumonia-like organisms in pure culture and in infected tissue culture cells. J Bacteriol. 1960 Feb;79:267–276. doi: 10.1128/jb.79.2.267-276.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREUNDT E. A. Morphology and classification of the PPLO. Ann N Y Acad Sci. 1960 Jan 15;79:312–325. doi: 10.1111/j.1749-6632.1960.tb42693.x. [DOI] [PubMed] [Google Scholar]
- GOODBURN G. M., MARMION B. P. A study of the properties of Eaton's primary atypical pneumonia organism. J Gen Microbiol. 1962 Oct;29:271–290. doi: 10.1099/00221287-29-2-271. [DOI] [PubMed] [Google Scholar]
- LIEBOW A. A., STEER A., BILLINGSLEY J. G. DESQUAMATIVE INTERSTITIAL PNEUMONIA. Am J Med. 1965 Sep;39:369–404. doi: 10.1016/0002-9343(65)90206-8. [DOI] [PubMed] [Google Scholar]
- LIU C. Studies on primary atypical pneumonia. I. Localization, isolation, and cultivation of a virus in chick embryos. J Exp Med. 1957 Oct 1;106(4):455–466. doi: 10.1084/jem.106.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lutsky I. I., Organick A. B. Pneumonia due to mycoplasma in gnotobiotic mice. I. Pathogenicity of Mycoplasma pneumoniae, Mycoplasma salivarium, and Mycoplasma pulmonis for the lungs of conventional and gnotobiotic mice. J Bacteriol. 1966 Oct;92(4):1154–1163. doi: 10.1128/jb.92.4.1154-1163.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson J. B., Lyons M. J. Phase-contrast and electron microscopy of murine strains of Mycoplasma. J Bacteriol. 1965 Dec;90(6):1750–1763. doi: 10.1128/jb.90.6.1750-1763.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]