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GST-Fyn-SH3-SH2 binding assay using high-speed CE. (A) Sample contained 0.1–2 μM GST-Fyn-SH3-SH2, 200 nM Fluor-Fyn peptide and 10 nM rhodamine 110. (B) Sample contained 0.5 μM GST-Fyn-SH3-SH2, 50–700 nM Fluor-Fyn peptide and 10 nM rhodamine 110. Separation conditions were the same as in Figure 4.
