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. 1966 Nov;92(5):1315–1320. doi: 10.1128/jb.92.5.1315-1320.1966

Enzymes in Glycolysis and the Citric Acid Cycle in the Yeast and Mycelial Forms of Paracoccidioides brasiliensis

Fuminori Kanetsuna 1, Luis M Carbonell 1
PMCID: PMC276425  PMID: 5924267

Abstract

Kanetsuna, Fuminori (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela), and Luis M. Carbonell. Enzymes in glycolysis and the citric acid cycle in the yeast and mycelial forms of Paracoccidioides brasiliensis. J. Bacteriol. 92:1315–1320. 1966.—Enzymatic activities in glycolysis, the hexose monophosphate shunt, and the citric acid cycle in cell-free extracts of the yeast and mycelial forms of Paracoccidioides brasiliensis were examined comparatively. Both forms have the enzymes of these pathways. Activities of glucose-6-phosphate dehydrogenase and malic dehydrogenase of the mycelial form were higher than those of the yeast form. Another 15 enzymatic activities of the mycelial form were lower than those of the yeast form. The activity of glyceraldehyde-3-phosphate dehydrogenase showed the most marked difference between the two forms, its activity in the mycelial form being about 20% of that in the yeast form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTNICKI GARCIA S. SYMPOSIUM ON BIOCHEMICAL BASES OF MORPHOGENESIS IN FUNGI. III. MOLD-YEAST DIMORPHISM OF MUCOR. Bacteriol Rev. 1963 Sep;27:293–304. doi: 10.1128/br.27.3.293-304.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernheim F. The Effect of Various Substances on the Oxygen Uptake of Blastomyces Dermatiditis. J Bacteriol. 1942 Nov;44(5):533–539. doi: 10.1128/jb.44.5.533-539.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DISCHE Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953 Oct;204(2):983–997. [PubMed] [Google Scholar]
  4. GALLANT J., STAPLETON R. PROPERTIES OF A TEMPERATURE-SENSITIVE REGULATORY SYSTEM. Proc Natl Acad Sci U S A. 1963 Aug;50:348–355. doi: 10.1073/pnas.50.2.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GILARDI G. L., LAFFER N. C. Nutritional studies on the yeast phase of Blastomyces dermatitidis and B. brasiliensis. J Bacteriol. 1962 Feb;83:219–227. doi: 10.1128/jb.83.2.219-227.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GREEN D. E., MII S., KOHOUT P. M. Studies on the terminal electron transport system. I. Succinic dehydrogenase. J Biol Chem. 1955 Dec;217(2):551–567. [PubMed] [Google Scholar]
  7. HORECKER B. L., SMYRNIOTIS P. Z. Phosphogluconic acid dehydrogenase from yeast. J Biol Chem. 1951 Nov;193(1):371–381. [PubMed] [Google Scholar]
  8. KORNBERG A., PRICER W. E., Jr Di- and triphosphopyridine nucleotide isocitric dehydrogenases in yeast. J Biol Chem. 1951 Mar;189(1):123–136. [PubMed] [Google Scholar]
  9. KORNBERG A., PRICER W. E., Jr Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside. J Biol Chem. 1951 Dec;193(2):481–495. [PubMed] [Google Scholar]
  10. KOVACHEVICH R., WOOD W. A. Carbohydrate metabolism by Pseudomonas fluorescens. III. Purification and properties of a 6-phosphogluconate dehydrase. J Biol Chem. 1955 Apr;213(2):745–756. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Levine S., Ordal Z. J. Factors Influencing the Morphology of Blastomyces dermatitidis. J Bacteriol. 1946 Dec;52(6):687–694. doi: 10.1128/jb.52.6.687-694.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MAHVI T. A. A COMPARATIVE STUDY OF THE YEAST AND MYCELIAL PHASES OF HISTOPLASMA CAPSULATUM. I. PATHWAYS OF CARBOHYDRATE DISSIMILATION. J Infect Dis. 1965 Jun;115:226–232. doi: 10.1093/infdis/115.3.226. [DOI] [PubMed] [Google Scholar]
  14. MASSEY V. The crystallization of fumarase. Biochem J. 1952 Jul;51(4):490–494. doi: 10.1042/bj0510490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  16. SMITH R. A., GUNSALUS I. C. Isocitritase; enzyme properties and reaction equilibrium. J Biol Chem. 1957 Nov;229(1):305–319. [PubMed] [Google Scholar]

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