Abstract
Hashimoto, Hajime (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. VII. Recombination of R factors with tetracycline-sensitive mutants. J. Bacteriol. 92:1351–1356. 1966.—The transmissible drug-resistance factor R is able to confer resistance to tetracycline (TC), chloramphenicol (CM), streptomycin (SM), and sulfonamide (SA) on a host bacterium when infected by cell-to-cell contact. Tetracycline-sensitive mutants were isolated from either CM- or SM-sensitive mutants of an R factor. Among 30 mutants isolated, 10 were point mutants which could recombine with each other, forming recombinant R factors able to grow on plates containing 50 μg/ml of TC. The recombination frequency of TC-resistant recombinants was 10−2 to 10−3 in bacterial cells carrying two types of TC-sensitive R factors by superinfection with both factors. Segregational patterns of the various markers on the R factor, i.e., chl, str, sul, and m, the locus determining R mating, and their linkage order, were investigated among TC-resistant recombinants of the R factor. When TC was used as the selective drug, the tet locus mapped on the R factor as an end marker. In view of the fact that these results are inconsistent with the linkage order of various markers reported previously, a circular genetic structure for the R factor which includes five tet-s and three chl-s loci is presented.
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Selected References
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- CAMPBELL A. Segregants from lysogenic heterogenotes carrying recombinant lambda prophages. Virology. 1963 Jun;20:344–356. doi: 10.1016/0042-6822(63)90124-7. [DOI] [PubMed] [Google Scholar]
- EDGAR R. S., LIELAUSIS I. TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4D: THEIR ISOLATION AND GENETIC CHARACTERIZATION. Genetics. 1964 Apr;49:649–662. doi: 10.1093/genetics/49.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hashimoto H., Hirota Y. Gene recombination and segregation of resistance factor R in Escherichia coli. J Bacteriol. 1966 Jan;91(1):51–62. doi: 10.1128/jb.91.1.51-62.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOB F., WOLLMAN E. L. Analyse des groupes de liaison génétique de différentes souches donatrices d'Escherichia coli K 12. C R Hebd Seances Acad Sci. 1957 Nov 18;245(21):1840–1843. [PubMed] [Google Scholar]
- KAISER A. D. A genetic study of the temperate coliphage. Virology. 1955 Nov;1(4):424–443. doi: 10.1016/0042-6822(55)90036-2. [DOI] [PubMed] [Google Scholar]
- MITSUHASHI S., HARADA K., HASHIMOTO H., KAMEDA M., SUZUKI M. Combination of two types of transmissible drug-resistance factors in a host bacterium. J Bacteriol. 1962 Jul;84:9–16. doi: 10.1128/jb.84.1.9-16.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUGINO Y., HIROTA Y. Conjugal fertility associated with resistance factor R in Escherichia coli. J Bacteriol. 1962 Nov;84:902–910. doi: 10.1128/jb.84.5.902-910.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]