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. 1966 Nov;92(5):1357–1363. doi: 10.1128/jb.92.5.1357-1363.1966

Conditions Affecting Transformation of a Group H Streptococcus

Harvey J Schlissel 1, C P Sword 1
PMCID: PMC276431  PMID: 4958880

Abstract

Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357–1363. 1966.—A defined transforming medium (DTM) containing buffer and 5 to 10 μg per ml of deoxyribonucleic acid was developed to study the physical and chemical requirements for optimal transformation in streptococcal strain SBE. Optimal transformation in DTM occurred at pH 7.5 and 7.0 in 0.07 m sodium phosphate buffer and 0.05 m tris(hydroxymethyl)aminomethane buffer, respectively. In the presence of either a monovalent or a divalent cation, transformation was stimulated maximally by Mn+2 (10−3m) and K+ (0.05 m). Other cations tested (Na+, Mg+2, Ca+2) were less stimulatory. A mixture of K+ and Mn+2 stimulated transformation to a level higher than either cation alone. Kinetic studies showed that the stimulating effect of cations was greatest during the early part of the transformation reaction and decreased with time. Transformation was inhibited by Cu+2 (10−5m) and Mn+2 (10−2m). Ethylenediaminetetraacetic acid (EDTA) inhibited transformation at 10−5m. The inhibition by EDTA could be overcome by Mn+2 during the early part of the transformation reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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