Abstract
Sparling, Philip F. (Communicable Disease Center, Atlanta, Ga.). Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance. J. Bacteriol. 92:1364–1371. 1966.—Eight strains of Neisseria gonorrhoeae were transformed to streptomycin resistance by deoxyribonucleic acid (DNA) extracted from a streptomycin-resistant strain of N. gonorrhoeae. In all strains, competence was greatest in the naturally occurring, virulent clonal types 1 and 2, which gave transformation frequencies up to 1%. Clonal types 3 and 4, which arise on laboratory transfer and are avirulent, gave maximal transformation frequencies of 0.00005%. Competence was maximal in lag and early log phases of growth, but was maintained throughout the growth cycle. A complex broth was required for the physiological expression of competence. The kinetics of DNA uptake, dose-response curve of DNA versus transformants, time required for phenotypic expression, and other features were similar to those in other bacterial transformation systems.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALEXANDER H. E., REDMAN W. Transformation of type specificity of meningococci; change in heritable type induced by type-specific extracts containing desoxyribonucleic acid. J Exp Med. 1953 Jun;97(6):797–806. doi: 10.1084/jem.97.6.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CATLIN B. W., CUNNINGHAM L. S. Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. J Gen Microbiol. 1961 Oct;26:303–312. doi: 10.1099/00221287-26-2-303. [DOI] [PubMed] [Google Scholar]
- DEACON W. E., PEACOCK W. L., Jr, FREEMAN E. M., HARRIS A. Identification of Neisseria gonorrhoeae by means of fluorescent antibodies. Proc Soc Exp Biol Med. 1959 Jun;101(2):322–325. doi: 10.3181/00379727-101-24925. [DOI] [PubMed] [Google Scholar]
- GERHARDT P., HEDEN C. G. Concentrated culture of gonococci in clear liquid medium. Proc Soc Exp Biol Med. 1960 Oct;105:49–51. doi: 10.3181/00379727-105-26005. [DOI] [PubMed] [Google Scholar]
- GOODGAL S. H., HERRIOTT R. M. Studies on transformations of Hemophilus influenzae. I. Competence. J Gen Physiol. 1961 Jul;44:1201–1227. doi: 10.1085/jgp.44.6.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hotchkiss R. D. CYCLICAL BEHAVIOR IN PNEUMOCOCCAL GROWTH AND TRANSFORMABILITY OCCASIONED BY ENVIRONMENTAL CHANGES. Proc Natl Acad Sci U S A. 1954 Feb;40(2):49–55. doi: 10.1073/pnas.40.2.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JYSSUM K., LIE S. GENETIC FACTORS DETERMINING COMPETENCE IN TRANSFORMATION OF NEISSERIA MENINGITIDIS. 1. A PERMANENT LOSS OF COMPETENCE. Acta Pathol Microbiol Scand. 1965;63:306–316. doi: 10.1111/apm.1965.63.2.306. [DOI] [PubMed] [Google Scholar]
- JYSSUM K., LIE S. TRANSFORMATION OF AUXOTROPHS OF NEISSERIA MENINGITIDIS. Acta Pathol Microbiol Scand. 1965;63:445–455. doi: 10.1111/apm.1965.63.3.445. [DOI] [PubMed] [Google Scholar]
- KAY D. The deoxyribonuclease of typhoid bacilli and its effect on typhoid bacteriophage nucleic acid. J Gen Microbiol. 1954 Aug;11(1):45–51. doi: 10.1099/00221287-11-1-45. [DOI] [PubMed] [Google Scholar]
- KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIE S. STUDIES ON THE PHENOTYPIC EXPRESSION OF COMPETENCE IN NEISSERIA MENINGITIDIS. Acta Pathol Microbiol Scand. 1965;64:119–129. doi: 10.1111/apm.1965.64.1.119. [DOI] [PubMed] [Google Scholar]
- Pakula R. Factors regulating competence in transformation of streptococci. J Bacteriol. 1965 Nov;90(5):1320–1324. doi: 10.1128/jb.90.5.1320-1324.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAVIN A. W. The genetics of transformation. Adv Genet. 1961;10:61–163. doi: 10.1016/s0065-2660(08)60116-9. [DOI] [PubMed] [Google Scholar]
- RAVIN A. W. The significance of bacterial transformations to studies of the neoplastic process. Ann N Y Acad Sci. 1957 Oct 21;68(2):335–348. doi: 10.1111/j.1749-6632.1957.tb56089.x. [DOI] [PubMed] [Google Scholar]
- Spencer H. T., Herriott R. M. Development of competence of Haemophilus influenzae. J Bacteriol. 1965 Oct;90(4):911–920. doi: 10.1128/jb.90.4.911-920.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]