Abstract
Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling β-d-galactosidase synthesis from Hfr and F′ donors of Escherichia coli. J. Bacteriol. 92:1378–1382. 1966.—Comparisons were made between Hfr1 and F13 donors with respect to the frequency of transfer and incorporation of genes controlling β-d-galactosidase synthesis. The Hfr1 donor transfers these genes as part of the chromosome, and the F13 donor transfers them by F-duction. The criterion used for gene transfer was the acquisition by recipient cells of the ability to synthesize the enzyme, β-d-galactosidase, measured by fluorogenic assays at the single-cell level. The criterion for incorporation was the formation of lac+ recombinant colonies. It was found that the two types of donor showed the same frequency of gene transfer, but the probability of incorporation was 10-fold higher in F13 matings than in Hfr1 matings. In the former, between 46 and 97% of the merozygotes produced recombinant colonies; in the latter, 2 to 6% did so.
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