Abstract
Jahiel, R. I. (Cornell University Medical College, New York, N.Y.), and E. D. Kilbourne. Reduction in plaque size and reduction in plaque number as differing indices of influenza virus-antibody reactions. J. Bacteriol. 92:1521–1534. 1966.—The serological reactivity of an antigenically hybrid influenza virus recombinant (X-7) was studied in a heteroploid cell plaquing system in which antisera to the parental viruses NWS/(A0) and RI/5+ (A2) were incorporated in agar overlay media. Two different effects on plaque formation were found. With NWS antiserum, there was close relationship between reduction in plaque size and in plaque number [plaque inhibition (PI) pattern]. With RI/5 antiserum, plaque size reduction (PSR) occurred over a wide zone of serum dilutions without concomitant change in plaque number (PSR pattern). Several different preinoculation neutralization tests showed a strong reactivity of X-7 with NWS antisera and little if any reactivity with RI/5 antisera. We interpret the differing effects of NWS and RI/5 antisera on X-7 as indicative of the possible occurrence of different mechanisms of neutralization or of the possible participation of different surface antigens. The kinetics of PSR are consistent with the hypothesis that it results from the reaction of RI/5 antiserum with the RI/5-like neuraminidase of X-7. Studies with the antiserum-in-overlay technique of PSR and PI patterns comprise a sensitive method for quantitative antigenic analysis of plaque-forming influenza viruses.
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