Abstract
The isolation of a rec− Hfr strain of Escherichia coli K-12 is described. The method used consisted of mating AB2463 F− Rec− His− Lac− with P4X6 Hfr Rec+ His+ Lac+, selecting Rec− His− Lac+ recombinants, and searching for Hfr strains. One Hfr rec− strain, no. 12, was used as donor in crosses with Rec+ and Rec− recipients. Crosses with Rec+ recipients are fertile, and those with Rec− recipients are almost infertile, the frequency of recombinants being 10−2 to 10−3 that found with Rec+ recipients. The Rec− mutant marker is transfered to and integrated into Rec+ recipients. Zygotic induction of prophage λ is observed in crosses between two Rec− strains. In crosses of F− Rec− with Hfr Rec−, the gradient of integration frequencies for markers progressively more distant from the origin is steeper than in the Rec+ × Rec− or the Rec− × Rec+ crosses.
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Selected References
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