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. 1967 Feb;93(2):605–617. doi: 10.1128/jb.93.2.605-617.1967

Kinetics of Growth of Individual Cells of Escherichia coli and Azotobacter agilis

R J Harvey 1, Allen G Marr 1, P R Painter 1
PMCID: PMC276485  PMID: 5335966

Abstract

Escherichia coli and Azotobacter agilis were grown in minimal media until a steady state was established. The distribution of cell size was determined electronically. From the equation of Collins and Richmond, the growth rate of individual cells was computed as a function of size. The main features of the growth of individual E. coli and A. agilis cells revealed by this work were: the specific growth rate decreased at the time of division, and both the absolute and specific growth rates increased between divisions. The frequency function of interdivision times was computed and was found to be positively skewed with a coefficient of variation of approximately 0.3. The results supported the hypothesis of Koch and Schaechter that the size of an individual cell at division is highly regulated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL A. Synchronization of cell division. Bacteriol Rev. 1957 Dec;21(4):263–272. doi: 10.1128/br.21.4.263-272.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COLLINS J. F., RICHMOND M. H. Rate of growth of Bacillus cereus between divisions. J Gen Microbiol. 1962 Apr;28:15–33. doi: 10.1099/00221287-28-1-15. [DOI] [PubMed] [Google Scholar]
  3. ERRINGTON F. P., POWELL E. O., THOMPSON N. GROWTH CHARACTERISITICS OF SOME GRAM-NEGATIVE BACTERIA. J Gen Microbiol. 1965 Apr;39:109–123. doi: 10.1099/00221287-39-1-109. [DOI] [PubMed] [Google Scholar]
  4. HELMSTETTER C. E., CUMMINGS D. J. AN IMPROVED METHOD FOR THE SELECTION OF BACTERIAL CELLS AT DIVISION. Biochim Biophys Acta. 1964 Mar 16;82:608–610. doi: 10.1016/0304-4165(64)90453-2. [DOI] [PubMed] [Google Scholar]
  5. Harvey R. J., Marr A. G. Measurement of size distributions of bacterial cells. J Bacteriol. 1966 Oct;92(4):805–811. doi: 10.1128/jb.92.4.805-811.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KOCH A. L., SCHAECHTER M. A model for statistics of the cell division process. J Gen Microbiol. 1962 Nov;29:435–454. doi: 10.1099/00221287-29-3-435. [DOI] [PubMed] [Google Scholar]
  7. KUBITSCHEK H. E. Normal distribution of cell generation rate. Exp Cell Res. 1962 Mar;26:439–450. doi: 10.1016/0014-4827(62)90150-7. [DOI] [PubMed] [Google Scholar]
  8. Knaysi G. A Morphological Study of Streptococcus fecalis. J Bacteriol. 1941 Nov;42(5):575–586. doi: 10.1128/jb.42.5.575-586.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knaysi G. A Photomicrographic Study of the Rate of Growth of Some Yeasts and Bacteria. J Bacteriol. 1940 Aug;40(2):247–253. doi: 10.1128/jb.40.2.247-253.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MITCHISON J. M. The growth of single cells. III. Streptococcus faecalis. Exp Cell Res. 1961 Jan;22:208–225. doi: 10.1016/0014-4827(61)90099-4. [DOI] [PubMed] [Google Scholar]
  11. Marr A. G., Harvey R. J., Trentini W. C. Growth and division of Escherichia coli. J Bacteriol. 1966 Jun;91(6):2388–2389. doi: 10.1128/jb.91.6.2388-2389.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. POWELL E. O. A NOTE ON KOCH AND SCHAECHTER'S HYPOTHESIS ABOUT GROWTH AND FISSION OF BACTERIA. J Gen Microbiol. 1964 Nov;37:231–249. doi: 10.1099/00221287-37-2-231. [DOI] [PubMed] [Google Scholar]
  13. SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]

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