Abstract
The antibiotic sensitivity of the individual organisms of a bacterial population was determined to study the comparative rates of development of resistance of Staphylococcus aureus to novobiocin, tetracycline, and to a combination of these antibiotics. Serial subculture of S. aureus with the combination of novobiocin-tetracycline (N-T 2.5:1; the ratio in serum of patients dosed with Panalba) showed a significant retardation of resistance outgrowth compared with subculture in the presence of the antibiotics individually. Increase in organisms resistant to novobiocin seen after one N-T subculture was related to the “concentration gap” between novobiocin and tetracycline. Two additional subcultures with N-T caused little or no increase in organisms resistant to novobiocin, tetracycline, or to the combination. The data suggest that the retardation of further development of resistance was the result of tetracycline inhibition of novobiocin-resistant strains and vice versa.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dowling H. F. Present status of therapy with combinations of antibiotics. Am J Med. 1965 Nov;39(5):796–811. doi: 10.1016/0002-9343(65)90098-7. [DOI] [PubMed] [Google Scholar]
- FINLAND M., WILCOX C. Antibiotic combinations and resistance to antibiotics: penicillin with other antibiotics against penicillin-resistant staphylococci. Proc Soc Exp Biol Med. 1953 Jul;83(3):605–608. doi: 10.3181/00379727-83-20433. [DOI] [PubMed] [Google Scholar]
- Gal K. Combined antibiotic therapy. Can Med Assoc J. 1965 Oct 16;93(16):844–847. [PMC free article] [PubMed] [Google Scholar]
- Garrod L. P. Antibiotic mixtures. S Afr Med J. 1965 Jul 24;39(27):607–610. [PubMed] [Google Scholar]
- PURCELL E. M., WRIGHT S. S., FINLAND M. Antibiotic combinations and resistance to antibiotics; penicillin-erythromycin and streptomycin-erythromycin combinations in vitro. Proc Soc Exp Biol Med. 1953 Jan;82(1):124–131. doi: 10.3181/00379727-82-20042. [DOI] [PubMed] [Google Scholar]
- WRIGHT S. S., PURCELL E. M., WILCOX C., BRODERICK M. K., FINLAND M. Antibiotic combinations and resistance to antibiotics; development of resistance during repeated subcultures of Staphylococci and certain Streptococci on media containing penicillin, streptomycin, erythromycin, terramycin, and chloramphenicol used singly and in pairs. J Lab Clin Med. 1953 Dec;42(6):877–895. [PubMed] [Google Scholar]
- WRIGHT S. S., PURCELL E. M., WILCOX C., BRODERICK M. K., FINLAND M. Antibiotic combinations and resistance: response of E. coli to antibiotics, singly and in pairs. Proc Soc Exp Biol Med. 1954 Jan;85(1):128–133. doi: 10.3181/00379727-85-20807. [DOI] [PubMed] [Google Scholar]