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. 1967 Mar;93(3):811–815. doi: 10.1128/jb.93.3.811-815.1967

Fate of Thymine-containing Dimers in the Deoxyribonucleic Acid of Ultraviolet-irradiated Bacillus subtilis

Robert C Shuster a,1
PMCID: PMC276522  PMID: 4960923

Abstract

The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UVR) and an ultraviolet light-sensitive (UVS) mutant. During incubation in the dark, dimers were excised from the DNA of the UVRB. subtilis, but remained in the DNA of the UVS mutant. About 40% of the excised dimers recovered in the wild type were in the acid-soluble fraction; the remainder were in the incubation medium. A UVS mutant of Escherichia coli K-12, shown previously to be defective in dimer excision, was irradiated with ultraviolet light and incubated under visible light for 3 hr. About 65% of thymine-containing photoproducts were removed from the DNA. These photoproducts were not recovered in the acid-soluble fraction. In comparison, the UVS mutant of B. subtilis lost only 13% of such photoproducts from DNA when exposed to light under the same conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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