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. 1967 Mar;93(3):811–815. doi: 10.1128/jb.93.3.811-815.1967

Fate of Thymine-containing Dimers in the Deoxyribonucleic Acid of Ultraviolet-irradiated Bacillus subtilis

Robert C Shuster a,1
PMCID: PMC276522  PMID: 4960923

Abstract

The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UVR) and an ultraviolet light-sensitive (UVS) mutant. During incubation in the dark, dimers were excised from the DNA of the UVRB. subtilis, but remained in the DNA of the UVS mutant. About 40% of the excised dimers recovered in the wild type were in the acid-soluble fraction; the remainder were in the incubation medium. A UVS mutant of Escherichia coli K-12, shown previously to be defective in dimer excision, was irradiated with ultraviolet light and incubated under visible light for 3 hr. About 65% of thymine-containing photoproducts were removed from the DNA. These photoproducts were not recovered in the acid-soluble fraction. In comparison, the UVS mutant of B. subtilis lost only 13% of such photoproducts from DNA when exposed to light under the same conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boling M. E., Setlow J. K. The resistance of Micrococcus radiodurans to ultraviolet radiation. 3. A repair mechanism. Biochim Biophys Acta. 1966 Jul 20;123(1):26–33. doi: 10.1016/0005-2787(66)90155-9. [DOI] [PubMed] [Google Scholar]
  3. ELLISON S. A., FEINER R. R., HILL R. F. A host effect on bacteriophage survival after ultraviolet irradiation. Virology. 1960 May;11:294–296. doi: 10.1016/0042-6822(60)90069-6. [DOI] [PubMed] [Google Scholar]
  4. HILL R. F., SIMSON E. A study of radiosensitive and radioresistant mutants of Escherichia coli strain B. J Gen Microbiol. 1961 Jan;24:1–14. doi: 10.1099/00221287-24-1-1. [DOI] [PubMed] [Google Scholar]
  5. HOWARD-FLANDERS P., BOYCE R. P., SIMSON E., THERIOT L. A genetic locus in E. coli K12 that controls the reactivation of UV-photoproducts associated with thymine in DNA. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2109–2115. doi: 10.1073/pnas.48.12.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RORSCH A., EDELMAN A., COHEN J. A. The gene-controlled radiation sensitivity in Escherichia coli. Biochim Biophys Acta. 1963 Feb 26;68:263–270. doi: 10.1016/0006-3002(63)90141-0. [DOI] [PubMed] [Google Scholar]
  8. RUPERT C. S. Photoreactivation of transforming DNA by an enzyme from bakers' yeast. J Gen Physiol. 1960 Jan;43:573–595. doi: 10.1085/jgp.43.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Riklis E. Studies on mechanism of repair of ultraviolet-irradiated viral and bacterial DNAin vivo and in vitro. Can J Biochem. 1965 Jul;43(7):1207–1219. doi: 10.1139/o65-132. [DOI] [PubMed] [Google Scholar]
  10. SAUERBIER W. The influence of the metabolic state of the host bacteria on the survival of phage T1 irradiated with ultraviolet light. Virology. 1962 May;17:164–170. doi: 10.1016/0042-6822(62)90093-4. [DOI] [PubMed] [Google Scholar]
  11. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Searashi T., Strauss B. Relation of the repair of damage induced by a monofunctional alkylating agent to the repair of damage induced by ultraviolet light in Bacillus subtilis. Biochem Biophys Res Commun. 1965 Sep 22;20(6):680–687. doi: 10.1016/0006-291x(65)90069-0. [DOI] [PubMed] [Google Scholar]
  14. Setlow R. B., Carrier W. L., Bollum F. J. Pyrimidine dimers in UV-irradiated poly dI:dC. Proc Natl Acad Sci U S A. 1965 May;53(5):1111–1118. doi: 10.1073/pnas.53.5.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Setlow R. B., Carrier W. L. Pyrimidine dimers in ultraviolet-irradiated DNA's. J Mol Biol. 1966 May;17(1):237–254. doi: 10.1016/s0022-2836(66)80105-5. [DOI] [PubMed] [Google Scholar]
  16. Strauss B., Searashi T., Robbins M. Repair of DNA studied with a nuclease specific for UV-induced lesions. Proc Natl Acad Sci U S A. 1966 Sep;56(3):932–939. doi: 10.1073/pnas.56.3.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TSUBOI K. K., PRICE T. D. Isolation, detection and measure of microgram quantities of labeled tissue nucleotides. Arch Biochem Biophys. 1959 Mar;81(1):223–237. doi: 10.1016/0003-9861(59)90192-4. [DOI] [PubMed] [Google Scholar]
  18. WITKIN E. M. Modification of mutagenesis initiated by ultraviolet light through postteatment of bacteria with basic dyes. J Cell Comp Physiol. 1961 Dec;58(3):135–144. doi: 10.1002/jcp.1030580413. [DOI] [PubMed] [Google Scholar]
  19. WULFF D. L., RUPERT C. S. Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast. Biochem Biophys Res Commun. 1962 Apr 20;7:237–240. doi: 10.1016/0006-291x(62)90181-x. [DOI] [PubMed] [Google Scholar]

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