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. Author manuscript; available in PMC: 2010 Mar 24.
Published in final edited form as: Biochemistry. 2009 Mar 24;48(11):2411–2421. doi: 10.1021/bi8021526

Figure 3. Functional Effects of Covalent Modification of Porcine SERCA2a in Cardiac Sarcoplasmic Reticulum.

Figure 3

Calcium-dependent ATPase activities (open symbols) or stoichiometries of fluorophore (FITC, panel A; IAF, panel B) labeling (closed symbols) were measured for cardiac SR microsomes in the absence (squares, circles) or presence (triangles) of 6 mM AMPPCP to block access to the nucleotide binding cleft. Specific labeling of Lys514, by FITC, or Cys674, by IAF in SERCA2a of cardiac SR membranes was performed and calcium-dependent ATPase activities were measured (25°C) as described in Materials and Methods.