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. 1967 Apr;93(4):1236–1241. doi: 10.1128/jb.93.4.1236-1241.1967

Drug Resistance of Enteric Bacteria VIII. Chromosomal Location of Nontransferable R Factor in Escherichia coli

Kenji Harada 1, Mitsuo Kameda 1, Mitsue Suzuki 1, Susumu Shigehara 1, Susumu Mitsuhashi 1
PMCID: PMC276591  PMID: 5340305

Abstract

The R21(TC) factor, obtained by transduction of the R10(TC.CM.SM.SA) factor with phage ε to group E Salmonella, is not transferable by the normal conjugal process. However, when R21(TC)+ transductants are infected with the F13 factor, the nontransferable R21(TC) factor acquires transmissibility by conjugation. R21(TC)+ conjugants of Escherichia coli K-12, to which only the R21(TC) factor was transmitted by cell-to-cell contact from an F′ R+ donor, were still unable to transfer their R21(TC) factor by conjugation. In crosses between Hfr and F−E. coli K-12 strains containing R21(TC), the gene responsible for tetracycline resistance was located on the E. coli K-12 chromosome between lac and pro, near lac.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HARADA K., KAMEDA M., SUZUKI M., EGAWA R., MITSUHASHI S. Studies on the drug-resistance of enteric bacteria. 10. Relation between transmissible drug-resistance (R) factor and fertility (F) factor in E. coli strain K-12. Jpn J Exp Med. 1961 Aug;31:291–299. [PubMed] [Google Scholar]
  4. HARADA K., KAMEDA M., SUZUKI M., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. 3. ACQUISITION OF TRANSFERABILITY OF NONTRANSMISSIBLE R(TC) FACTOR IN COOPERATION WITH F FACTOR AND FORMATION OF FR(TC). J Bacteriol. 1964 Nov;88:1257–1265. doi: 10.1128/jb.88.5.1257-1265.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HARADA K., KAMEDA M., SUZUKI M., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON. J Bacteriol. 1963 Dec;86:1332–1338. doi: 10.1128/jb.86.6.1332-1338.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARADA K., KAMEDA M., SUZUKI M., MITSUHASHI S. MUTANT OF SALMONELLA PHAGE EPSILON 34 WITH LOSS OF CONVERTING ABILITY. Jpn J Microbiol. 1964 Dec;8:125–130. doi: 10.1111/j.1348-0421.1964.tb00265.x. [DOI] [PubMed] [Google Scholar]
  7. HERSHEY A. D., MELECHEN N. E. Synthesis of phage-precursor nucleic acid in the presence of chloramphenicol. Virology. 1957 Feb;3(1):207–236. doi: 10.1016/0042-6822(57)90034-x. [DOI] [PubMed] [Google Scholar]
  8. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  10. Mitsuhashi S. Transmissible drug-ressistance factor R with special reference to replication. Gunma J Med Sci. 1965 Dec;14(4):245–257. [PubMed] [Google Scholar]
  11. SUGINO Y., HIROTA Y. Conjugal fertility associated with resistance factor R in Escherichia coli. J Bacteriol. 1962 Nov;84:902–910. doi: 10.1128/jb.84.5.902-910.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SUZUKI A., KAWANISHI T. [Studies on the aerobic spore forming bacteria from skim milk powder. II. On the heat-resistance and pathogenicity to experimental animals of Bacillus cereus from skim milk powder]. Eisei Shikenjo Hokoku. 1961 Sep;79:201–205. [PubMed] [Google Scholar]
  13. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J Bacteriol. 1962 Apr;83:727–735. doi: 10.1128/jb.83.4.727-735.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduotion of resistance factors. J Bacteriol. 1961 Aug;82:202–209. doi: 10.1128/jb.82.2.202-209.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

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