Abstract
Mycobacterium tuberculosis has been found to exhibit autolysis and spontaneous secondary growth in modified Sauton medium. The phenomenon is a function of high glycerol concentration of the medium and limitation of air in test tubes. It is not a function of depletion of nutrients and is probably not a manifestation of lysogeny or spheroplast formation. The cycle of growth, autolysis, and secondary growth is related to development of lethal conditions during a period of severe oxygen limitation followed by a metabolic change associated with slower depletion of glycerol.
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Selected References
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- BLOCH H., SEGAL W. Biochemical differentiation of Mycobacterium tuberculosis grown in vivo and in vitro. J Bacteriol. 1956 Aug;72(2):132–141. doi: 10.1128/jb.72.2.132-141.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowles J. A., Segal W. Kinetics of Utilization of Organic Compounds in the Growth of Mycobacterium tuberculosis. J Bacteriol. 1965 Jul;90(1):157–163. doi: 10.1128/jb.90.1.157-163.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FISHER M. W., KIRCHHEIMER W. F. Studies on the growth of Mycobacteria. I. The occurrence of arithmetic linear growth. Am Rev Tuberc. 1952 Dec;66(6):758–761. doi: 10.1164/art.1952.66.6.758. [DOI] [PubMed] [Google Scholar]
- GREENWALT T. J., STEANE E. A. THE MEASUREMENT OF GLYCEROL IN THE PRESENCE OF CITRATE. Transfusion. 1964 May-Jun;4:206–209. doi: 10.1111/j.1537-2995.1964.tb02860.x. [DOI] [PubMed] [Google Scholar]
- KOCH-WESER D., EBERT R. H. Tetrazolium reduction by tubercle bacilli. J Lab Clin Med. 1955 Oct;46(4):608–614. [PubMed] [Google Scholar]
- REDMOND W. B., BOWMAN B. U., Jr Studies on autolysis of mycobacteria, the effect of temperature change. J Bacteriol. 1955 Mar;69(3):293–297. doi: 10.1128/jb.69.3.293-297.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUSSELL R. L., JANN G. J., FROMAN S. Lysogeny in the mycobacteria. 1. The establishment of lysogeny. Am Rev Respir Dis. 1960 Sep;82:384–393. doi: 10.1164/arrd.1960.82.3.384. [DOI] [PubMed] [Google Scholar]
- SCHAEFER W. B., MARSHAK A., BURKHART B. The growth of Mycobacterium tuberculosis as a function of its nutrients. J Bacteriol. 1949 Nov;58(5):549–563. doi: 10.1128/jb.58.5.549-563.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THACORE H., WILLETT H. P. FORMATION OF SPHEROPLASTS OF MYCOBACTERIUM TUBERCULOSIS BY LYSOZYME. Proc Soc Exp Biol Med. 1963 Oct;114:43–47. doi: 10.3181/00379727-114-28580. [DOI] [PubMed] [Google Scholar]
- WHITE A., KNIGHT V. Effect of tween 80 and serum on the interaction of mycobacteriophage D-29 with certain mycobacterial species. Am Rev Tuberc. 1958 Jan;77(1):134–145. doi: 10.1164/artpd.1958.77.1.134. [DOI] [PubMed] [Google Scholar]